5α-reductase type 3 expression in human benign and malignant tissues: A comparative analysis during prostate cancer progression
| Autor: | Godoy, Alejandro, Kawinski, Elzbieta, Li, Yun, Oka, Daizo, Alexiev, Borislav, Azzouni, Faris, Titus, Mark A., Mohler, James L. |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Reverse Transcriptase Polymerase Chain Reaction Blotting Western Prostate Prostatic Hyperplasia Prostatic Neoplasms Enzyme-Linked Immunosorbent Assay Epithelial Cells Immunohistochemistry Article Isoenzymes 3-Oxo-5-alpha-Steroid 4-Dehydrogenase Cell Line Tumor Disease Progression Tumor Cells Cultured Humans RNA Messenger Muscle Skeletal |
| DOI: | 10.17615/djmq-qj85 |
| Popis: | A third isozyme of human 5α-steroid reductase, 5α-reductase-3, was identified in prostate tissue at the mRNA level. However, the levels of 5α-reductase-3 protein expression and its cellular localization in human tissues remain unknown.A specific monoclonal antibody was developed, validated, and used to characterize for the first time the expression of 5α-reductase-3 protein in 18 benign and 26 malignant human tissue types using immunostaining analyses.In benign tissues, 5α-reductase-3 immunostaining was high in conventional androgen-regulated human tissues, such as skeletal muscle and prostate. However, high levels of expression also were observed in non-conventional androgen-regulated tissues, which suggest either multiples target tissues for androgens or different functions of 5α-reductase-3 among human tissues. In malignant tissues, 5α-reductase-3 immunostaining was ubiquitous but particularly over-expressed in some cancers compared to their benign counterparts, which suggests a potential role for 5α-reductase-3 as a biomarker of malignancy. In benign prostate, 5α-reductase-3 immunostaining was localized to basal epithelial cells, with no immunostaining observed in secretory/luminal epithelial cells. In high-grade prostatic intraepithelial neoplasia (HGPIN), 5α-reductase-3 immunostaining was localized in both basal epithelial cells and neoplastic epithelial cells characteristic of HGPIN. In androgen-stimulated and castration-recurrent prostate cancer (CaP), 5α-reductase-3 immunostaining was present in most epithelial cells and at similar levels, and at levels higher than observed in benign prostate. Analyses of expression and functionality of 5α-reductase-3 in human tissues may prove useful for development of treatment for benign prostatic enlargement and prevention and treatment of CaP. |
| Databáze: | OpenAIRE |
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