Internally standardized amino acid analysis for determining peptide/carrier protein coupling ratio
| Autor: | Xi Lin, Youling Wu, Jonglin Tsao, Henry Lackland, Stanley Stein, Guillermo Tous |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Aminoisobutyric Acids
Ovalbumin Molecular Sequence Data Biophysics Peptide Biochemistry chemistry.chemical_compound Ethyldimethylaminopropyl Carbodiimide Peptide synthesis Amino Acid Sequence Cysteine Amino Acids Molecular Biology Peptide sequence Carbodiimide chemistry.chemical_classification Chromatography Edman degradation Cell Biology Reference Standards Amino acid chemistry Hemocyanins Chromatography Gel 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Carrier Proteins Peptides Conjugate |
| Zdroj: | Analytical biochemistry. 197(1) |
| ISSN: | 0003-2697 |
| Popis: | A method based on amino acid analysis has been developed for monitoring the covalent conjugation of synthetic peptide haptens to carrier proteins. The marker amino acid, alpha-aminobutyric acid, is included in the sequence during peptide synthesis. Following reaction, the carrier protein-conjugate is freed of excess peptide by two successive rounds of gel filtration chromatography. Amino acid analysis of a hydrolysate of the conjugate allows the calculation of the coupling ratio of the peptide to the carrier protein. Two typical procedures for conjugation, carbodiimide cross-linking and cysteine-thiol reaction with maleimidyl-proteins, have been evaluated. |
| Databáze: | OpenAIRE |
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