Simple screening method for molds producing intracellular mycotoxins in pure cultures
| Autor: | O Filtenborg, J C Frisvad, J A Svendsen |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Microbiological Techniques
food.ingredient medicine.disease_cause Applied Microbiology and Biotechnology chemistry.chemical_compound food Mold medicine Agar Mycotoxin Mycelium Roquefortine C Chromatography Ecology biology Penicillium Mycotoxins biology.organism_classification Aspergillus chemistry Penitrem A Chromatography Thin Layer Research Article Food Science Biotechnology Sterigmatocystin |
| Zdroj: | Applied and Environmental Microbiology. 45:581-585 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.45.2.581-585.1983 |
| Popis: | A simple screening method for molds producing the intracellular mycotoxins brevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem A, roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegnin was developed. After removing an agar plug from the mold culture, the mycelium on the plug is wetted with a drop of methanol-chloroform (1:2). By this treatment the intracellular mycotoxins are extracted within seconds and transferred directly to a thin-layer chromatography plate by immediately placing the plug on the plate while the mycelium is still wet. After removal of the plug, known thin-layer chromatographic procedures are carried out. The substrate (Czapek yeast autolysate agar) and growth conditions (25 degrees C for 7 days) used by Penicillium taxonomists proved suitable for the production of the mycotoxins investigated when 60 known toxigenic isolates and 865 cultures isolated from foods and feedstuffs were tested with this screening method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|