ESCRT-I components of the endocytic machinery are required for Rim101-dependent ambient pH regulation in the yeast Yarrowia lipolytica
| Autor: | Grégory Da Costa, Claude Gaillardin, Sylvie Blanchin-Roland |
|---|---|
| Přispěvatelé: | Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Institut National Agronomique Paris Grignon (INAPG) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Endocytic cycle Molecular Sequence Data Vesicular Transport Proteins Yarrowia Endosomes macromolecular substances Endocytosis Microbiology ESCRT Insertional mutagenesis Fungal Proteins MULTIVESICULAR BODY 03 medical and health sciences Gene Expression Regulation Fungal Transport Vesicles Gene 030304 developmental biology 0303 health sciences biology Endosomal Sorting Complexes Required for Transport ENDOSOME 030306 microbiology Nuclear Proteins Sequence Analysis DNA Hydrogen-Ion Concentration biology.organism_classification Yeast YARROWIA LIPOLYTICA PH REGULATION ESCRT complex DNA-Binding Proteins ESCRT-I [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry MVB Mutation ALKALINE REPONSE |
| Zdroj: | Microbiology Microbiology, Microbiology Society, 2005, 151 (Pt 11), pp.3627-3637. ⟨10.1099/mic.0.28196-0⟩ |
| ISSN: | 1350-0872 1465-2080 |
| DOI: | 10.1099/mic.0.28196-0⟩ |
| Popis: | Ambient pH signalling involves a cascade of conserved Rim or Pal products in ascomycetous yeasts or filamentous fungi, respectively. Insertional mutagenesis in the yeastYarrowia lipolyticaidentified two components of the endosome-associated ESCRT-I complex involved in multivesicular body (MVB) vesicle formation, YlVps28p and YlVps23p. They were shown to be required at alkaline pH, like Rim factors, for transcriptional activation of alkaline-induced genes and repression of acid-induced genes. The constitutively activeYlRIM101-1119allele, which suppresses the pH-signalling defects ofYlrimmutations, also suppressesYlvpsdefects in pH response, but not in endocytosis. The contribution of the ESCRT-III component Snf7p could not be assessed due to the essential nature of this component inY. lipolytica. Unlike Rim factors, YlVps4p, a component of the MVB pathway acting downstream from ESCRT complexes, seems not to be required for the alkaline response. InY. lipolytica, allvpsmutations including those affectingYlVPS4, affected growth at acidic pH, a feature not exhibited byYlrimmutations. These results suggest that Rim and Vps pathways cooperate in ambient pH signalling and that this relation is conserved across the full range of hemiascomycetous yeasts. |
| Databáze: | OpenAIRE |
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