Tendon Cell Regeneration Is Mediated by Attachment Site-Resident Progenitors and BMP Signaling
| Autor: | Xubo Niu, Arul Subramanian, Thomas F. Schilling, Tyler H. Hwang, Jenna L. Galloway |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biology General Biochemistry Genetics and Molecular Biology Animals Genetically Modified Tendons 03 medical and health sciences 0302 clinical medicine Tendon cell Animals Regeneration Perichondrium Progenitor cell Musculoskeletal System Zebrafish Progenitor Stem Cells Regeneration (biology) Scleraxis Cell Differentiation biology.organism_classification Embryonic stem cell Cell biology 030104 developmental biology Bone Morphogenetic Proteins embryonic structures General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | Current Biology. 30:3277-3292.e5 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2020.06.016 |
| Popis: | The musculoskeletal system is a striking example of how cell identity and position is coordinated across multiple tissues to ensure function. However, it is unclear upon tissue loss, such as complete loss of cells of a central musculoskeletal connecting tendon, whether neighboring tissues harbor progenitors capable of mediating regeneration. Here, using a zebrafish model, we genetically ablate all embryonic tendon cells and find complete regeneration of tendon structure and pattern. We identify two regenerative progenitor populations, sox10+ perichondrial cells surrounding cartilage and nkx2.5+ cells surrounding muscle. Surprisingly, laser ablation of sox10+ cells, but not nkx2.5+ cells, increases tendon progenitor number in the perichondrium, suggesting a mechanism to regulate attachment location. We find BMP signaling is active in regenerating progenitor cells and is necessary and sufficient for generating new scxa+ cells. Our work shows that muscle and cartilage connective tissues harbor progenitor cells capable of fully regenerating tendons, and this process is regulated by BMP signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|