A Combination Strategy of Solubility Enhancers for Effective Production of Soluble and Bioactive Human Enterokinase
| Autor: | Yiseul Ryu, Seungmin Kim, Jinhak Kwon, Joong-jae Lee, Hyeongjun Cho |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Enteropeptidase
Chemistry Protein digestion Recombinant Fusion Proteins Bioengineering General Medicine Trypsin Applied Microbiology and Biotechnology Fusion protein Recombinant Proteins law.invention Solubility Biochemistry law Escherichia coli Recombinant DNA medicine Humans Amino Acid Sequence Thioredoxin Enhancer Peptide sequence Biotechnology medicine.drug |
| DOI: | 10.1101/2021.07.22.453328 |
| Popis: | Enterokinase is one of the hydrolases that catalyze hydrolysis to regulate biological processes in intestinal visceral mucosa. Enterokinase plays an essential role in accelerating the process of protein digestion as it converts trypsinogen into active trypsin by accurately recognizing and cleaving a specific peptide sequence, (Asp)4-Lys. Due to its exceptional substrate specificity, enterokinase is widely used as a versatile molecular tool in various bioprocessing, especially in removing fusion tags from recombinant proteins. Despite its biotechnological importance, mass production of soluble enterokinase in bacteria still remains an unsolved challenge. Here, we present an effective production strategy of human enterokinase using tandemly linked solubility enhancers consisting of thioredoxin, phosphoglycerate kinase or maltose-binding protein. The resulting enterokinases exhibited significantly enhanced solubility and bacterial expression level while retaining enzymatic activity, which demonstrates that combinatorial design of fusion proteins has the potential to provide an efficient way to produce recombinant proteins in bacteria. |
| Databáze: | OpenAIRE |
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