European Sero-Epidemiology Network: standardisation of the results of diphtheria antitoxin assays
| Autor: | Guy A. M. Berbers, Nick Andrews, F. Fievet-Groyne, A. Tischer, P. A. C. Maple, M Raux, C. von Hunolstein, R.M Ölander, Henrik Aggerbeck |
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| Rok vydání: | 2000 |
| Předmět: |
Diphtheria antitoxin
Enzyme-Linked Immunosorbent Assay medicine.disease_cause Neutralization Serology 03 medical and health sciences 0302 clinical medicine Neutralization Tests Immunity Chlorocebus aethiops medicine Animals Humans 030212 general & internal medicine Vero Cells Immunoassay Diphtheria toxin 0303 health sciences General Veterinary General Immunology and Microbiology biology 030306 microbiology Toxin business.industry Diphtheria Public Health Environmental and Occupational Health medicine.disease Virology Diphtheria Antitoxin 3. Good health Infectious Diseases Immunology biology.protein Regression Analysis Molecular Medicine Antibody business |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0264-410X |
| Popis: | A European Sero-Epidemiological Network (ESEN) was established with the aim to co-ordinate and harmonise serological surveillance of immunity to communicable diseases in Europe. In this study the inter-laboratory standardisation of diphtheria toxin antibody measurements is reported. A standard panel of 162 sera was tested by the participating laboratories using an in vitro assay of their choice: VERO cell toxin neutralisation assay (NT), double-antigen delayed time-resolved fluorescence immuno-assay (DA-DELFIA), double-antigen enzyme-linked immunosorbent assay (DAE), toxin binding inhibition test (ToBI) and an indirect enzyme-linked immunosorbent assay (ELISA). The results were standardised using regression against the NT. The variations due to inter-laboratory and inter-assay variation, which would otherwise make it difficult directly to compare the main serum bank results by the different laboratories and the various assays were successfully minimised by the standardisation. The regression equations obtained will be used to transform the respective local results of testing the main serum bank into the reference test unitages. This study also gave the opportunity to compare the various assays within and between laboratories. This demonstrated a very high correlation between DA-DELFIA, DAE, ToBI and the NT. The ELISA showed a good correlation, too, however sera below some 0.1 IU/ml were overestimated. |
| Databáze: | OpenAIRE |
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