Spinach consumption ameliorates the gut microbiota and dislipaemia in rats with diet-induced non-alcoholic fatty liver disease (NAFLD)
| Autor: | U. Vrhovsek, D. Masuero, Laura Inés Elvira-Torales, N. Hidalgo, E. Soini, María Jesús Periago, Rocío González-Barrio, Carlos Gómez-Gallego, F. J. García-Alonso, Inmaculada Navarro-González |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Settore CHIM/01 - CHIMICA ANALITICA medicine.medical_specialty Normal diet Blood lipids Gut microbiota Gut flora Diet High-Fat Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Non-alcoholic Fatty Liver Disease Spinacia oleracea Internal medicine Lactobacillus medicine Animals Humans Metabolomics 030109 nutrition & dietetics Bacteria biology Chemistry Cholesterol Fatty liver food and beverages Cholesterol LDL General Medicine Fatty Acids Volatile Lipid Metabolism biology.organism_classification medicine.disease Gastrointestinal Microbiome Rats 030104 developmental biology Endocrinology Liver Spinach Steatosis Food Science |
| Zdroj: | Food & Function. 10:2148-2160 |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | Non-alcoholic fatty liver disease (NAFLD) is defined as the accumulation of fat in liver cells, which causes serious health consequences. Animal and human studies suggest that the gut microbiota plays a role in the pathogenesis of NAFLD. Here, we investigated whether spinach consumption could ameliorate high-fat-diet-induced disturbances in certain intestinal bacterial groups and products derived from their metabolism, such as short-chain fatty acids (SCFAs) and microbial phenolic catabolites. Attention is also paid to blood lipids and glucose. In the study, a rat model of high-fat-diet-induced NAFLD was used. There were six experimental groups: NC (normal diet), NB (normal diet + 2.5% spinach), NA (normal diet + 5% spinach), HC (high-fat diet), HB (high-fat diet + 2.5% spinach) and HA (high-fat diet + 5% spinach). The rats consumed these diets for five weeks, and after that, they were sacrificed and plasma, urine, intestinal content, faeces and liver samples were taken. Biochemical parameters were analyzed in plasma, phenolic catabolites were quantified in the faeces, urine, plasma and liver by UPLC-ESI-MS/MS, and the analysis of the microbiota and SCFAs in the intestinal content was performed by qPCR and GLC. Consumption of a high-fat diet caused NAFLD and dislipaemia and altered the gut microbiota and the pattern of SCFAs and phenolic gut microbial catabolites. Supplementation with spinach partially ameliorated some alterations induced by the high-fat diet, in particular by increasing the Lactobacillus counts, reducing the fasting glucose and total and LDL-cholesterol and preventing excess liver cholesterol accumulation, thereby improving the values of the steatosis biomarkers. |
| Databáze: | OpenAIRE |
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