Cloning and expression of the first anaerobic toxin gene from Clostridium bifermentans subsp. malaysia, encoding a new mosquitocidal protein with homologies to Bacillus thuringiensis delta-endotoxins
| Autor: | Armelle Delécluse, Marguerite-M. Lecadet, Frédérique Barloy, Luc Nicolas |
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| Rok vydání: | 1996 |
| Předmět: |
Mosquito Control
Bacterial Toxins Molecular Sequence Data Aedes aegypti Microbiology Hemolysin Proteins Clostridium Bacterial Proteins Bacillus thuringiensis parasitic diseases Animals Amino Acid Sequence Cloning Molecular Molecular Biology Gene Peptide sequence Anopheles stephensi Clostridium bifermentans Bacillus thuringiensis Toxins Base Sequence biology fungi biology.organism_classification Endotoxins Culex genomic DNA Research Article |
| Zdroj: | Journal of Bacteriology. 178:3099-3105 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.178.11.3099-3105.1996 |
| Popis: | A gene (cbm71) encoding a 71,128-Da mosquitocidal protein (Cbm71) was obtained by screening a size-fractionated XbaI digest of total genomic DNA from Clostridium bifermentans subsp. malaysia CH18 with two gene-specific oligonucleotide probes. The sequence of the Cbm71 protein, as deduced from the sequence of cbm71, corresponds to that of the 66-kDa protein previously described as one of the mosquitocidal components of C. bifermentans subsp. malaysia. Cbm71 shows limited similarities with Bacillus thuringiensis delta-endotoxins, especially in the four first conserved blocks. However, Cbm71 was not immunologically related to any of the Cry toxins and thus belongs to a novel class of mosquitocidal protein. The cbm71 gene was expressed in a nontoxic strain of B. thuringiensis, and Cbm71 was produced during sporulation and secreted to the supernatant of culture. Trichloroacetic-precipitated supernatant preparations were toxic for mosquito larvae of the species Aedes aegypti, Culex pipiens, and Anopheles stephensi. |
| Databáze: | OpenAIRE |
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