PPAR-γ Promotes Hematoma Clearance through Haptoglobin-Hemoglobin-CD163 in a Rat Model of Intracerebral Hemorrhage

Autor: Fang Xue, Xin-gang Sun, Shu-na Duan, Tong Li, Liang Dong, Gaiqing Wang
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Agonist
Male
medicine.medical_specialty
Article Subject
medicine.drug_class
Antigens
Differentiation
Myelomonocytic
Neurosciences. Biological psychiatry. Neuropsychiatry
Receptors
Cell Surface
Rats
Sprague-Dawley
03 medical and health sciences
Hemoglobins
0302 clinical medicine
Hematoma
Antigens
CD
Internal medicine
Edema
medicine
Animals
cardiovascular diseases
Cerebral Hemorrhage
Intracerebral hemorrhage
Neurons
biology
Haptoglobins
business.industry
Haptoglobin
Antagonist
Brain
General Medicine
medicine.disease
Rats
PPAR gamma
Disease Models
Animal
030104 developmental biology
Neuropsychology and Physiological Psychology
Endocrinology
Neurology
Brain Injuries
biology.protein
Neurology (clinical)
Hemoglobin
medicine.symptom
business
CD163
030217 neurology & neurosurgery
RC321-571
Research Article
Zdroj: Behavioural Neurology
Behavioural Neurology, Vol 2018 (2018)
ISSN: 1875-8584
0953-4180
Popis: Background and Purpose. PPAR-γ is a transcriptional factor which is associated with promoting hematoma clearance and reducing neurological dysfunction after intracerebral hemorrhage (ICH). Haptoglobin- (Hp-) hemoglobin- (Hb-) CD163 acts as a main pathway to Hb scavenging after ICH. The effect of PPAR-γ on the Hp-Hb-CD163 signaling pathway has not been reported. We hypothesized that PPAR-γ might protect against ICH-induced neuronal injury via activating the Hp-Hb-CD163 pathway in a rat ICH model. Methods. 107 Sprague-Dawley rats were used in this research. They were randomly allocated to 4 groups as follows: sham group, vehicle group, monascin-treated group, and Glivec-treated group. Animals were euthanized at 3 days after the model was established successfully. We observed the effects of PPAR-γ on the brain water content, hemoglobin levels, and the expressions of CD163 and Hp in Western blot and real-ime PCR; meanwhile, we measured hematoma volumes and edema areas by MRI scanning. Result. The results showed that PPAR-γ agonist significantly reduced hematoma volume, brain edema, and hemoglobin after ICH. It also enhanced CD163 and Hp expression while PPAR-γ antagonist had the opposite effects. Conclusions. PPAR-γ promotes hematoma clearance and plays a protective role through the Hp-Hb-CD163 pathway in a rat collagenase infusion ICH model.
Databáze: OpenAIRE
Externí odkaz: