Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway
| Autor: | Isabelle Léger-Silvestre, Sylvie Camier, Nicole Gas, Olga Koroleva, Anne-Marie Dechampesme |
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| Rok vydání: | 1999 |
| Předmět: |
Ribosomal Proteins
Cytoplasm Saccharomyces cerevisiae Proteins pre-rRNA Genes Fungal 5.8S ribosomal RNA Gene Expression yeasts Saccharomyces cerevisiae Biology ribosomes Fungal Proteins Preribosomal RNA RNA Precursors RNA polymerase I RNA Processing Post-Transcriptional RRNA processing Cell Nucleus RNA Ribosomal 5S Intron RNA-Binding Proteins RNA RNA Fungal Cell Biology 5S ribosomal RNA Ribosomal RNA Molecular biology Cell biology Molecular Weight Kinetics RNA Ribosomal RNA editing Mutation cell nucleolus Nucleic Acid Conformation Regular Articles |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.145.7.1369 |
| Popis: | A collection of yeast strains surviving with mutant 5S RNA has been constructed. The mutant strains presented alterations of the nucleolar structure, with less granular component, and a delocalization of the 25S rRNA throughout the nucleoplasm. The 5S RNA mutations affected helix I and resulted in decreased amounts of stable 5S RNA and of the ribosomal 60S subunits. The shortage of 60S subunits was due to a specific defect in the processing of the 27SB precursor RNA that gives rise to the mature 25S and 5.8S rRNA. The processing rate of the 27SB pre-rRNA was specifically delayed, whereas the 27SA and 20S pre-rRNA were processed at a normal rate. The defect was partially corrected by increasing the amount of mutant 5S RNA. We propose that the 5S RNA is recruited by the pre-60S particle and that its recruitment is necessary for the efficient processing of the 27SB RNA precursor. Such a mechanism could ensure that all newly formed mature 60S subunits contain stoichiometric amounts of the three rRNA components. |
| Databáze: | OpenAIRE |
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