A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances
| Autor: | Yonatan Katz, Heinz Beck, Gal Elyasaf, Ana Parabucki, Ilan Lampl, Daniel Müller-Komorowska |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Nervous system
Computer science Physiology Action Potentials Electrode Recording Molecular neuroscience Nervous System Mice Animal Cells Current clamp Medicine and Health Sciences Biology (General) Membrane Electrophysiology Neurons Measurement Ecology Electrophysiology Laboratory Equipment medicine.anatomical_structure Bioassays and Physiological Analysis Computational Theory and Mathematics Modeling and Simulation Physical Sciences Excitatory postsynaptic potential Engineering and Technology Single trial Experimental methods Cellular Types Anatomy Electrical Engineering Research Article QH301-705.5 Models Neurological Materials Science Material Properties Capacitance Neurophysiology Equipment Inhibitory postsynaptic potential Research and Analysis Methods Membrane Potential Cellular and Molecular Neuroscience medicine Genetics Animals CA1 Region Hippocampal Molecular Biology Ecology Evolution Behavior and Systematics Pipettes Electrophysiological Techniques Computational Biology Biology and Life Sciences Cell Biology Cellular Neuroscience Synapses Neuroscience Electrical Circuits |
| Zdroj: | PLoS Computational Biology PLoS Computational Biology, Vol 17, Iss 12, p e1009725 (2021) |
| ISSN: | 1553-7358 |
| DOI: | 10.1371/journal.pcbi.1009725 |
| Popis: | The firing of neurons throughout the brain is determined by the precise relations between excitatory and inhibitory inputs, and disruption of their balance underlies many psychiatric diseases. Whether or not these inputs covary over time or between repeated stimuli remains unclear due to the lack of experimental methods for measuring both inputs simultaneously. We developed a new analytical framework for instantaneous and simultaneous measurements of both the excitatory and inhibitory neuronal inputs during a single trial under current clamp recording. This can be achieved by injecting a current composed of two high frequency sinusoidal components followed by analytical extraction of the conductances. We demonstrate the ability of this method to measure both inputs in a single trial under realistic recording constraints and from morphologically realistic CA1 pyramidal model cells. Future experimental implementation of our new method will facilitate the understanding of fundamental questions about the health and disease of the nervous system. Author summary Most neurons in the brain receive synaptic inputs from both excitatory and inhibitory neurons. Together, these inputs determine neuronal activity: excitatory synapses shift the electrical potential across the membrane towards the threshold for generation of action potentials, whereas inhibitory synapses lower this potential away from the threshold. Action potentials are the rapid electrochemical signals that transmit information to other neurons and they are critical for the information processing abilities of the brain. Although there are many ways to measure either excitatory or inhibitory inputs, these methods have been unable to measure both at the same time. Measuring both inputs together is essential towards understanding how neurons integrate information. We developed a new analytical method to measure excitatory and inhibitory inputs at the same time from the voltage response to injection of an alternating current into a neuron. We describe the foundation of this new method and find that it works in biologically realistic simulations of neurons. By using this technique in real neurons, scientists could investigate basic principles of information processing in the healthy and diseased brain. |
| Databáze: | OpenAIRE |
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