Genetic diversity of Theileria orientalis in tick vectors detected in Hokkaido and Okinawa, Japan
| Autor: | Hisashi Inokuma, Shirou Matsui, Thillaiampalam Sivakumar, Mamoru Oshiro, Hiroshi Hata, Ikuo Igarashi, Yasuhiro Kuroda, Kotaro Matsumoto, Yukio Nakamura, Hidenari Yamashina, Satoshi Zakimi, Seiji Kondo, Naoya Kojima, Naomi Ota, Naoaki Yokoyama, Natsuko Fukumoto |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Male Genotype Blotting Western Protozoan Proteins Zoology Antibodies Protozoan Cattle Diseases Antigens Protozoan Ixodes persulcatus Tick Microbiology Polymerase Chain Reaction Ticks Japan Theileria parasitic diseases Genetics Animals Tick Control Molecular Biology Ecology Evolution Behavior and Systematics Genetic diversity biology Ecology Genetic Variation bacterial infections and mycoses Haemaphysalis biology.organism_classification Recombinant Proteins Theileriasis Infectious Diseases Arachnid Vectors Cattle Female Haemaphysalis longicornis |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 12(8) |
| ISSN: | 1567-7257 |
| Popis: | In the present study, we investigated the possible tick vectors that can transmit Theileria orientalis in eastern Hokkaido, Japan. Questing ticks collected from three different districts, Taiki, Otofuke, and Shin-Hidaka, of Hokkaido included Ixodes persulcatus, Haemaphysalis megaspinosa, Haemaphysalis douglasi, and Ixodes ovatus, while all the ticks collected from Yonaguni island of Okinawa were identified as Haemaphysalis longicornis. When the ticks were screened by polymerase chain reaction (PCR) for T. orientalis, the parasite was commonly detected among all tick species. Genotype-specific PCR assays revealed that all tick species in Hokkaido were predominantly detected with type 2, while ticks collected from Okinawa (H. longicornis) were predominantly detected with type 1. Consistent with the genetic diversity of T. orientalis in ticks, genotyping PCR assays from cattle grazed in the same Hokkaido sampling locations identified type 2 as the most prevalent genotype. This study provides the first identification of I. persulcatus, H. megaspinosa, H. douglasi, and I. ovatus as possible tick vectors of T. orientalis, and finds that the variety of vectors apparently capable of transmitting T. orientalis is wider in Japan than expected. The authors suggest that tick control strategies should be modified in Hokkaido based on the seasonal activities of ticks identified in the present study. |
| Databáze: | OpenAIRE |
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