Recombination between immunoglobulin variable region gene segments is enhanced by transcription
| Autor: | Erik Selsing, Mark W. Moore, T K Blackwell, George D. Yancopoulos, Frederick W. Alt, Heikyung Suh, Stuart Lutzker |
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| Rok vydání: | 1986 |
| Předmět: |
Recombination
Genetic B-Lymphocytes Multidisciplinary Transcription Genetic Immunoglobulin Variable Region TCF4 Biology Transfection Molecular biology Germline Cell Line Enhancer Elements Genetic Gene Expression Regulation Transcription (biology) Cell culture Gene expression biology.protein Animals Antibody Receptor Gene |
| Zdroj: | Nature. 324:585-589 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/324585a0 |
| Popis: | Immunoglobulin (Ig) variable (V) region genes are assembled in precursor B (pre-B) lymphocytes from multiple germline segments. The heavy-chain V-region gene is composed of variable (VH), diversity (D) and joining (JH) segments; kappa (K) and lambda (lambda) light-chain V-region genes have analogous VL and JL segments. Assembly of Ig V-gene segments, as well as those of the highly related T-cell receptor, is regulated at several levels and shows both stage and tissue specificity; for example Ig heavy-chain V-gene assembly precedes that of Ig light chains during B-cell differentiation. Joining of all classes of V-gene segments involves conserved recognition sequences that are probably targets for a common recombinase. Evidence has been presented suggesting that rearrangement of specific classes of segments is regulated by modulation of their accessibility to the recombinase. To elucidate mechanisms which control V-region gene assembly, we have investigated the effect of flanking gene expression on the frequency at which introduced V-gene segments are assembled in pre-B cell lines. Our findings suggest that transcription may play a direct role in the regulation of immunoglobulin V-gene assembly. |
| Databáze: | OpenAIRE |
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