Toward real-time virtual biopsy of oral lesions using confocal laser endomicroscopy interfaced with embedded computing
| Autor: | Patricia S. P. Thong, Stephanus S. Tandjung, Wei Ming Chiew, Malini Olivo, Hock Soon Seah, Kemao Qian, Khee Chee Soo, Feng Lin, Ramaswamy Bhuvaneswari, Muhammad Mobeen Movania |
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| Rok vydání: | 2012 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Computer science Confocal Biomedical Engineering Mice Nude Capsule Endoscopy Sensitivity and Specificity law.invention Biomaterials Mice User-Computer Interface Confocal microscopy law Computer Systems Biopsy Image Interpretation Computer-Assisted Endomicroscopy medicine Animals Humans Mouth neoplasm Mice Inbred BALB C Microscopy Confocal medicine.diagnostic_test business.industry Biopsy Needle Reproducibility of Results Volume rendering Signal Processing Computer-Assisted Laser Image Enhancement Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Surgery Computer-Assisted Embedded system Mouth Neoplasms business |
| Zdroj: | Journal of biomedical optics. 17(5) |
| ISSN: | 1560-2281 |
| Popis: | Oral lesions are conventionally diagnosed using white light endoscopy and histopathology. This can pose a challenge because the lesions may be difficult to visualise under white light illumination. Confocal laser endomicroscopy can be used for confocal fluorescence imaging of surface and subsurface cellular and tissue structures. To move toward real-time "virtual" biopsy of oral lesions, we interfaced an embedded computing system to a confocal laser endomicroscope to achieve a prototype three-dimensional (3-D) fluorescence imaging system. A field-programmable gated array computing platform was programmed to enable synchronization of cross-sectional image grabbing and Z-depth scanning, automate the acquisition of confocal image stacks and perform volume rendering. Fluorescence imaging of the human and murine oral cavities was carried out using the fluorescent dyes fluorescein sodium and hypericin. Volume rendering of cellular and tissue structures from the oral cavity demonstrate the potential of the system for 3-D fluorescence visualization of the oral cavity in real-time. We aim toward achieving a real-time virtual biopsy technique that can complement current diagnostic techniques and aid in targeted biopsy for better clinical outcomes. |
| Databáze: | OpenAIRE |
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