Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies
| Autor: | Kishore M. Paknikar, Reihaneh Haghniaz, Rinku D Umrani |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Melanoma
Experimental Pharmaceutical Science Apoptosis 02 engineering and technology 01 natural sciences Antioxidants International Journal of Nanomedicine Drug Discovery Tissue Distribution lanthanum strontium manganese oxide nanoparticles Heat-Shock Proteins Original Research biology Chemistry Caspase 3 Melanoma Hyperthermia Treatment Dextrans Oxides General Medicine 021001 nanoscience & nanotechnology Magnetic Resonance Imaging Catalase Caspases DNA fragmentation 0210 nano-technology MRI Hyperthermia Programmed cell death tumor regression Biophysics Bioengineering DNA Fragmentation 010402 general chemistry survival Biomaterials In vivo Lanthanum Heat shock protein medicine melanoma In Situ Nick-End Labeling Animals HSP70 Heat-Shock Proteins Cell Proliferation Inflammation Organic Chemistry Hyperthermia Induced medicine.disease 0104 chemical sciences Mice Inbred C57BL Manganese Compounds Strontium heat shock proteins Cancer research biology.protein Nanoparticles |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Reihaneh Haghniaz, Rinku D Umrani, Kishore M Paknikar Centre for Nanobioscience, Agharkar Research Institute, Pune, India Purpose: The aim of this study was to evaluate radiofrequency-induced dextran-coated lanthanum strontium manganese oxide nanoparticles-mediated hyperthermia to be used for tumor regression in mice.Materials and methods: Nanoparticles were injected intra-tumorally in melanoma-bearing C57BL/6J mice and were subjected to radiofrequency treatment.Results: Hyperthermia treatment significantly inhibited tumor growth (~84%), increased survival (~50%), and reduced tumor proliferation in mice. Histopathological examination demonstrated immense cell death in treated tumors. DNA fragmentation, increased terminal deoxynucleotidyl transferase-dUTP nick end labeling signal, and elevated levels of caspase-3 and caspase-6 suggested apoptotic cell death. Enhanced catalase activity suggested reactive oxygen species-mediated cell death. Enhanced expression of heat shock proteins 70 and 90 in treated tumors suggested the possible development of “antitumor immunity”.Conclusion: The dextran-coated lanthanum strontium manganese oxide-mediated hyperthermia can be used for the treatment of cancer. Keywords: lanthanum strontium manganese oxide nanoparticles, melanoma, tumor regression, survival, heat shock proteins, MRI |
| Databáze: | OpenAIRE |
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