Butyric acid, a gut bacteria metabolite, lowers arterial blood pressure via colon-vagus nerve signaling and GPR41/43 receptors
| Autor: | Aneta Sawicka, Marcin Ufnal, Ewa Koźniewska, Marta Gawrys-Kopczynska, Emilia Samborowska, Marta Aleksandrowicz, Maksymilian Onyszkiewicz, Piotr Konopelski |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Physiology Colon medicine.medical_treatment Clinical Biochemistry Hemodynamics 030204 cardiovascular system & hematology Pharmacology Butyric acid Receptors G-Protein-Coupled 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Heart Rate Physiology (medical) Integrative Physiology medicine Animals Arterial Pressure Rats Wistar Receptor Bacterial metabolites biology Chemistry Vagus Nerve Arteries Vagotomy SCFA Fatty Acids Volatile Vagus nerve Gastrointestinal Microbiome Rats Nitric oxide synthase 030104 developmental biology NG-Nitroarginine Methyl Ester Circulatory system biology.protein Blood pressure Hypotension Ex vivo Signal Transduction |
| Zdroj: | Pflugers Archiv |
| ISSN: | 1432-2013 0031-6768 |
| Popis: | Butyric acid (BA) is a short-chain fatty acid (SCFA) produced by gut bacteria in the colon. We hypothesized that colon-derived BA may affect hemodynamics. Arterial blood pressure (BP) and heart rate (HR) were recorded in anesthetized, male, 14-week-old Wistar rats. A vehicle, BA, or 3-hydroxybutyrate, an antagonist of SCFA receptors GPR41/43 (ANT) were administered intravenously (IV) or into the colon (IC). Reactivity of mesenteric (MA) and gracilis muscle (GMA) arteries was tested ex vivo. The concentration of BA in stools, urine, portal, and systemic blood was measured with liquid chromatography coupled with mass spectrometry. BA administered IV decreased BP with no significant effect on HR. The ANT reduced, whereas L-NAME, a nitric oxide synthase inhibitor, did not affect the hypotensive effect of BA. In comparison to BA administered intravenously, BA administered into the colon produced a significantly longer decrease in BP and a decrease in HR, which was associated with a 2–3-fold increase in BA colon content. Subphrenic vagotomy and IC pretreatment with the ANT significantly reduced the hypotensive effect. Ex vivo, BA dilated MA and GMA. In conclusion, an increase in the concentration of BA in the colon produces a significant hypotensive effect which depends on the afferent colonic vagus nerve signaling and GPR41/43 receptors. BA seems to be one of mediators between gut microbiota and the circulatory system. Electronic supplementary material The online version of this article (10.1007/s00424-019-02322-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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