Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection
| Autor: | Annemieke Madder, Joke Elskens |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
PROTEIN
OLIGONUCLEOTIDE Computational biology macromolecular substances 010402 general chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry LONG NONCODING RNAS chemistry.chemical_compound LINKING REACTION Molecular Biology 010405 organic chemistry Oligonucleotide Chemistry Disease mechanisms technology industry and agriculture Biology and Life Sciences DNA 0104 chemical sciences CAPTURE COUMARIN Nucleic Acid Probes Chemistry (miscellaneous) PHOTOAFFINITY PROBES Nucleic acid Biomarker (medicine) Identification (biology) COVALENT ABASIC SITES DUPLEX Overall efficiency |
| Zdroj: | RSC CHEMICAL BIOLOGY RSC Chemical Biology |
| ISSN: | 2633-0679 |
| Popis: | Understanding the intricate interaction pattern of nucleic acids with other molecules is essential to gain further insight in biological processes and disease mechanisms. To this end, a multitude of hybridization-based assays have been designed that rely on the non-covalent recognition between complementary nucleic acid sequences. However, the ephemeral nature of these interactions complicates straightforward analysis as low efficiency and specificity are rule rather than exception. By covalently locking nucleic acid interactions by means of a crosslinking agent, the overall efficiency, specificity and selectivity of hybridization-based assays could be increased. In this mini-review we highlight methodologies that exploit the use of crosslinker-modified nucleic acid probes for interstrand nucleic acid crosslinking with the objective to study, detect and identify important targets as well as nucleic acid sequences that can be considered relevant biomarkers. We emphasize on the usefulness and advantages of crosslinking agents and elaborate on the chemistry behind the crosslinking reactions they induce. Crosslinker-modified nucleic acid probes are promising substitutes for regular oligonucleotide probes in hybridization-based assays, as they allow a more selective and efficient detection of nucleic acid targets and nucleic acid biomarkers. |
| Databáze: | OpenAIRE |
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