An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay
| Autor: | Donghua Liu, Peter Firbas, Joanna Ślusarczyk, Aurel Popescu, Elena Bonciu, Alka Srivastava, Alessio Papini, Dmitry S. Pesnya, Cleiton Pereira de Souza, Silvia Schiff, Anca Nicoleta Sutan, Mehmet Cengiz Karaismailoglu, Felicia Menicucci, Carmem Silvia Fontanetti, Jiang Wu-sheng, Anton V. Romanovsky |
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| Přispěvatelé: | Belirlenecek, University of Craiova, Private Laboratory for Plant Cytogenetics, Universidade Estadual Paulista (Unesp), Tianjin Normal University, Istanbul University, University of Florence, Russian Academy of Sciences, University of Pitesti, Jan Kochanowski University, University of Lucknow |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine fertilizers Standardization macromolecular substances medicine.disease_cause 01 natural sciences 03 medical and health sciences herbicides Allium test cytotoxicity genotoxicity heavy metals pesticides radioactivity ultrastructure Genetics Agricultural and Biological Sciences (all) medicine Cytotoxicity Traditional medicine biology Research Subject Categories::NATURAL SCIENCES food and beverages Heavy metals biology.organism_classification 030104 developmental biology Allium General Agricultural and Biological Sciences Genotoxicity 010606 plant biology & botany |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2165-5391 0008-7114 |
| Popis: | Made available in DSpace on 2018-12-11T17:38:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-03 A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student’s t, and the non-parametric tests Kruskal–Wallis and Mann–Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug. Department of Agricultural and Forestry Technology University of Craiova Private Laboratory for Plant Cytogenetics Department of Biology UNESP (Universidade Estadual Paulista) Tianjin Key Laboratory of Animal and Plant Resistance College of Life Sciences Tianjin Normal University Department of Biology Faculty of Science Istanbul University Department of Chemistry “Ugo Schiff” University of Florence Laboratory of Experimental Ecology Papanin Institute for Biology of Inland Waters Russian Academy of Sciences Department of Natural Sciences University of Pitesti Department of Biology University of Florence Department of Ecology and Environmental Protection Jan Kochanowski University Department of Botany University of Lucknow Department of Biology UNESP (Universidade Estadual Paulista) |
| Databáze: | OpenAIRE |
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