Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells
| Autor: | Chen Huang, Jing Yan, Jing Zhang, Jiyu Miao, Ni Hou, Yun Feng, Yi Gao, Yani Chen, Huahua Zhang, Yu Wang, Wanjuan Xue, Juan Du, Yameng Wei, Ningning Xin, Haiyan Shi, Yongcheng Liu, Jiming Han, Dan Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Article Subject Colorectal cancer NEIL1 Apoptosis General Biochemistry Genetics and Molecular Biology DNA Glycosylases 03 medical and health sciences 0302 clinical medicine Transcription (biology) medicine Humans Viability assay RNA Neoplasm Cell Proliferation bcl-2-Associated X Protein General Immunology and Microbiology Cell growth Chemistry General Medicine Transfection medicine.disease HCT116 Cells digestive system diseases Caspase 9 MicroRNAs 030104 developmental biology HEK293 Cells Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Cancer research Medicine Signal transduction Colorectal Neoplasms Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 |
| Popis: | The study is aimed at investigating the role of Nei endonuclease VIII-like1 (NEIL1) in the pathogenesis of colorectal cancer (CRC). The human CRC (HCT116 and SW480) cells were subjected to the siRNA silencing and recombinant plasmid overexpression of NEIL1. Transfection of siNEIL1 significantly inhibited the cell growth. It also increased the Bax expression levels, while it decreased the Bcl-2 expression levels in human CRC cells, leading the Bax/Bcl-2 balance toward apoptosis. Moreover, the apoptosis was promoted through the caspase-9 signaling pathway. One the other hand, high expression of NEIL1 promoted the cell viability and reduced the apoptosis, inducing the balance of Bax/Bcl-2 in the human colon cancer cells to be antiapoptotic. In addition, the caspase-9 signaling pathway inhibited apoptosis, contrary to the results obtained by downregulating NEIL1 expression. Furthermore, NEIL1 was negatively regulated by miR-7-5p, indicating that miR-7-5p inhibited the NEIL1 expression after transcription. Overexpression of miR-7-5p reversed the effects of NEIL1 on these CRC cells. In conclusion, NEIL1 promotes the proliferation of CRC cells, which is regulated negatively by miR-7-5p. These findings suggest that NEIL1 is a potential therapeutic target for CRC. |
| Databáze: | OpenAIRE |
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