Yersinia-triggered arthritis in IL-12p40-deficient mice: relevant antigens and local expression of Toll-like receptor mRNA

Autor:	Silvia G. Correa, D. O. Castro, M. S. Di Genaro, Ricardo Javier Eliçabe, A. M. S. De Guzman, D. E. Cargnelutti, J. V. Gutiérrez
Rok vydání:	2007
Předmět:	Male Yersinia Infections Immunology Arthritis Gene Expression Spleen Yersinia Arthritis Reactive Mice Rheumatology medicine Immunology and Allergy Mesenteric lymph nodes Animals RNA Messenger Yersinia enterocolitica Mice Knockout Antigens Bacterial biology Interleukin-12 Subunit p40 Tumor Necrosis Factor-alpha Toll-Like Receptors Yersiniosis General Medicine biology.organism_classification medicine.disease Molecular biology Antibodies Bacterial Toll-Like Receptor 2 Mice Inbred C57BL Toll-Like Receptor 4 TLR2 medicine.anatomical_structure TLR4 Female Joints
Zdroj:	Scandinavian journal of rheumatology. 36(1)
ISSN:	0300-9742
Popis:	To study the role of IL-12p40 at the onset of reactive arthritis (ReA) after Yersinia enterocolitica O:3 infection, and analyse relevant microbial antigens and articular expression of Toll-like receptor (TLR) mRNA.Wild-type C57BL/6 and IL-12p40-deficient (IL-12p40-/-) mice were orogastrically infected with Y. enterocolitica O:3. Early (day 3) and late (day 21) after infection, the number of bacteria were determined in Peyer's patches (PP), mesenteric lymph nodes (MLN), the spleen, and joints. Histological studies of joints were performed. Collagen-specific and anti-Yersinia antibodies were measured by enzyme-linked immunosorbent assay (ELISA). The presence of Yersinia antigens was studied by dot blot. Induction of articular mRNA of TLR2, TLR4, and tumour necrosis factor (TNF)-alpha was analysed by reverse transcription-polymerase chain reaction (RT-PCR). TNFalpha protein levels were measured by ELISA.At day 3, bacterial recovery in PP, MLN, and spleen was significantly increased in IL-12p40-/- mice. Histopathological changes were observed in IL-12p40-/- mice at day 21 after infection, and correlated with higher antibody response against type II collagen. Although live bacteria could not be isolated at day 21 after infection, articular microbial components, especially from the outer membrane (OM), were detected. Moreover, intra-articular immunoglobulins to Yersinia antigens were significantly higher in IL-12p40-/- mice. Furthermore, mRNA levels for TLR2, TLR4 and TNFalpha, and TNFalpha protein were increased in joints from IL-12p40-/- mice.We concluded that IL-12p40 influences the resistance against Yersinia-triggered ReA. Bacterial products such as Yersinia OM could contribute to the ReA by induction of articular TLR expression, which results in an inflammatory response in the joint.
Databáze:	OpenAIRE
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