Deciduous DPSCs Ameliorate MPTP-Mediated Neurotoxicity, Sensorimotor Coordination and Olfactory Function in Parkinsonian Mice

Autor: Kasi Marimuthu, Sivachandran Parimannan, Christopher Simon, Manickam Ravichandran, Arulmoli Krishnan, Heera Rajandas, Nur Afiqah Mohamad, Jagadeesh Dhamodharan, Premasangery Kathivaloo, Bharathi Sengodan, Quan Fu Gan, Vasanth Raj Palanimuthu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Olfactory system
Male
Parkinson's disease
lcsh:Chemistry
chemistry.chemical_compound
Mice
tyrosine hydroxylase
lcsh:QH301-705.5
Cells
Cultured
Spectroscopy
Behavior
Animal
MPTP
Stem Cells
Dopaminergic
Cell Differentiation
Parkinson Disease
General Medicine
dental pulp stem cells
Computer Science Applications
Tyrosine 3-Monooxygenase
Substantia nigra
Article
Catalysis
Inorganic Chemistry
SDG 3 - Good Health and Well-being
stomatognathic system
medicine
Animals
Humans
intranasal delivery
Physical and Theoretical Chemistry
Pars Compacta
Molecular Biology
Dental Pulp
Tyrosine hydroxylase
Pars compacta
business.industry
Dopaminergic Neurons
Organic Chemistry
Neurotoxicity
MPTP Poisoning
medicine.disease
nervous system diseases
chemistry
nervous system
lcsh:Biology (General)
lcsh:QD1-999
behavioural analysis
Nerve Degeneration
Parkinson’s disease
business
Neuroscience
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 3
International Journal of Molecular Sciences, Vol 20, Iss 3, p 568 (2019)
Simon, C, Gan, Q F, Kathivaloo, P, Mohamad, N A, Dhamodharan, J, Krishnan, A, Sengodan, B, Palanimuthu, V R, Marimuthu, K, Rajandas, H, Ravichandran, M & Parimannan, S 2019, ' Deciduous DPSCs Ameliorate MPTP-Mediated Neurotoxicity, Sensorimotor Coordination and Olfactory Function in Parkinsonian Mice ', International journal of molecular sciences, vol. 20, no. 3, 568 . https://doi.org/10.3390/ijms20030568
ISSN: 1422-0067
DOI: 10.3390/ijms20030568
Popis: Parkinson&rsquo
s disease (PD) is a neurodegenerative disorder defined by progressive deterioration of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Dental pulp stem cells (DPSCs) have been proposed to replace the degenerated dopaminergic neurons due to its inherent neurogenic and regenerative potential. However, the effective delivery and homing of DPSCs within the lesioned brain has been one of the many obstacles faced in cell-based therapy of neurodegenerative disorders. We hypothesized that DPSCs, delivered intranasally, could circumvent these challenges. In the present study, we investigated the therapeutic efficacy of intranasally administered DPSCs in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. Human deciduous DPSCs were cultured, pre-labelled with PKH 26, and intranasally delivered into PD mice following MPTP treatment. Behavioural analyses were performed to measure olfactory function and sensorimotor coordination, while tyrosine hydroxylase (TH) immunofluorescence was used to evaluate MPTP neurotoxicity in SNpc neurons. Upon intranasal delivery, degenerated TH-positive neurons were ameliorated, while deterioration in behavioural performances was significantly enhanced. Thus, the intranasal approach enriched cell delivery to the brain, optimizing its therapeutic potential through its efficacious delivery and protection against dopaminergic neuron degeneration.
Databáze: OpenAIRE
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