Energy Transfer in Aminonaphthalimide-Boron-Dipyrromethene (BODIPY) Dyads upon One- and Two-Photon Excitation: Applications for Cellular Imaging
| Autor: | Daniel Collado, Pratik Sen, Patricia Remón, Yolanda Vida, Uwe Pischel, Francisco Najera, Ezequiel Perez-Inestrosa |
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| Rok vydání: | 2013 |
| Předmět: |
Boron Compounds
Fluorescence-lifetime imaging microscopy Photochemistry Biochemistry Two-photon absorption Fluorescence chemistry.chemical_compound Two-photon excitation microscopy Humans Fluorescent Dyes Photons Microscopy Confocal Molecular Structure Organic Chemistry General Chemistry Chromophore Acceptor Naphthalimides Microscopy Fluorescence Multiphoton Energy Transfer chemistry Quantum Theory BODIPY Excitation HeLa Cells |
| Zdroj: | Chemistry - An Asian Journal. 9:797-804 |
| ISSN: | 1861-4728 |
| DOI: | 10.1002/asia.201301334 |
| Popis: | Aminonaphthalimide-BODIPY energy transfer cassettes were found to show very fast (kEET ≈ 10(10)-10(11) s(-1) and efficient BODIPY fluorescence sensitization. This was observed upon one- and two-photon excitation, which extends the application range of the investigated bichromophoric dyads in terms of accessible excitation wavelengths. In comparison with the direct excitation of the BODIPY chromophore, the two-photon absorption cross-section δ of the dyads is significantly incremented by the presence of the aminonaphthalimide donor [δ ≈ 10 GM for the BODIPY versus 19-26 GM in the dyad at λ(exc)=840 nm; 1 GM (Goeppert-Mayer unit)=10(-50) cm(4) smolecule(-1) photon-(1)]. The electronic decoupling of the donor and acceptor, which is a precondition for the energy transfercassette concept, was demonstrated by time-dependent density functional theory calculations. The applicability of the new probes in the one- and twophoton excitation mode was demonstrated in a proof-of-principle approach in the fluorescence imaging of HeLa cells. To the best of our knowledge, this is the first demonstration of the merging of multiphoton excitation with the energy transfer cassette concept for a BODIPY-containing dyad. |
| Databáze: | OpenAIRE |
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