Dietary histidine, threonine, or taurine supplementation affects gilthead seabream (Sparus aurata) immune status
| Autor: | Benjamín Costas, Tomé S. Silva, L. Ramos-Pinto, Luís E.C. Conceição, Jaume Pérez-Sánchez, Jorge Dias, Marina Machado, Josep A. Calduch-Giner |
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| Přispěvatelé: | European Commission, Fundação para a Ciência e a Tecnologia (Portugal) |
| Rok vydání: | 2021 |
| Předmět: |
CLEC10A
Taurine medicine.medical_specialty Hematology General Veterinary Veterinary medicine Blood leucocytes Biology Mucus Immune-modulation Transcriptome chemistry.chemical_compound Endocrinology Immune system chemistry QL1-991 Mucosal immunity Internal medicine SF600-1100 medicine Amino acids Animal Science and Zoology Threonine Zoology Histidine |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Animals, Vol 11, Iss 1193, p 1193 (2021) Animals Volume 11 Issue 5 |
| Popis: | © 2021 by the authors. AAs have become interesting feed ingredients to be used in functional fish feeds as not only are they protein building blocks, but they also participate in several other key metabolic processes. In the present study, a comprehensive analysis of transcriptomics, hematology, and humoral immune parameters (plasma and skin mucus) were measured twice over the course of the feeding trial (four weeks). Plasma antiprotease activity increased in fish fed Thr compared to those fed the CTRL and Tau treatments, regardless of sampling time. The bactericidal activity in skin mucus decreased in fish fed Tau and His treatments compared to those fed the CTRL diet after two weeks. The membrane IgT (mIgT) was upregulated in fish fed Tau after four weeks, while C-type lectin domain family domain 10 member (clec10a) was downregulated in fish fed Thr after two weeks of feeding. By comparing the molecular signatures of head-kidney by means of a PLS-DA, it is possible to visualize that the main difference is between the two sampling points, regardless of diet. Altogether, these results suggest that dietary supplementation with these AAs at the tested levels causes mild immune-modulation effects in gilthead seabream, which should be further studied under disease challenge conditions. This work was partially supported by Projects ALISSA (reference ALG-01-0247- FEDER-3520), UIDB/04423/2020, UIDP/04423/2020 and IF/00482/2014, financed by Portugal and the European Union through FEDER, COMPETE 2020, and CRESC Algarve 2020, in the framework of Portugal 2020, and through the COMPETE and Operational Human Potential Programs and national funds through Fundação para a Ciência e a Tecnologia (FCT, Portugal). This study was also partially funded by project ATLANTIDA (ref. NORTE-01-0145-FEDER-000040), supported by the Norte Portugal Regional Operational Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement and through the European Regional Development Fund (ERDF). L. Ramos-Pinto, and B. Costas were supported by FCT, Portugal (PD/BDE/114436/2016 and IF/00197/2015, respectively). |
| Databáze: | OpenAIRE |
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