An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
| Autor: | Yao-Ping Shi, Zheng-Qian Bian, Wei-Jian Huang, Qian Liu, Wei-Jian Li, Zhen-Yu Wang, Hong-Ping Wu, Tian-Jie Yuan, Gong-Bo Fu, Wei-Feng Yu, Cai-Yang Chen, Bo Zhai, Hong-Dan Zhang, Min Zeng, Hong-Shu Jing, Xiao Shi, He-Xin Yan, Xue-Jing Zhu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Swine Pharmacology Extracorporeal law.invention 03 medical and health sciences 0302 clinical medicine law Albumins Medicine Animals Humans Hepatic encephalopathy business.industry Extracorporeal circulation Albumin Bioartificial liver device General Medicine Liver Failure Acute medicine.disease Liver Artificial Liver regeneration Hepatocyte nuclear factors 030104 developmental biology Liver Hepatic stellate cell Hepatocytes 030211 gastroenterology & hepatology business |
| Zdroj: | Science translational medicine. 12(551) |
| ISSN: | 1946-6242 |
| Popis: | Clinical advancement of the bioartificial liver is hampered by the lack of expandable human hepatocytes and appropriate bioreactors and carriers to encourage hepatic cells to function during extracorporeal circulation. We have recently developed an efficient approach for derivation of expandable liver progenitor-like cells from human primary hepatocytes (HepLPCs). Here, we generated immortalized and functionally enhanced HepLPCs by introducing FOXA3, a hepatocyte nuclear factor that enables potentially complete hepatic function. When cultured on macroporous carriers in an air-liquid interactive bioartificial liver (Ali-BAL) support device, the integrated cells were alternately exposed to aeration and nutrition and grew to form high-density three-dimensional constructs. This led to highly efficient mass transfer and supported liver functions such as albumin biosynthesis and ammonia detoxification via ureagenesis. In a porcine model of drug overdose-induced acute liver failure (ALF), extracorporeal Ali-BAL treatment for 3 hours prevented hepatic encephalopathy and led to markedly improved survival (83%, n = 6) compared to ALF control (17%, n = 6, P = 0.02) and device-only (no-cell) therapy (0%, n = 6, P = 0.003). The blood ammonia concentrations, as well as the biochemical and coagulation indices, were reduced in Ali-BAL-treated pigs. Ali-BAL treatment attenuated liver damage, ameliorated inflammation, and enhanced liver regeneration in the ALF porcine model and could be considered as a potential therapeutic avenue for patients with ALF. |
| Databáze: | OpenAIRE |
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