A Fermented Substance fromAspergillus phoenicisReduces Liver Fibrosis Induced by Carbon Tetrachloride in Rats

Autor: Hsun-Lang Fang, Jinn-Jsyy Lai, Wei-Lii Lin, Wen-Chuan Lin
Rok vydání: 2007
Předmět:
Male
Matrix metalloproteinase
Liver Cirrhosis
Experimental
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Lipid peroxidation
Random Allocation
chemistry.chemical_compound
Malondialdehyde
Carbon Tetrachloride
Reverse Transcriptase Polymerase Chain Reaction
Alanine Transaminase
Organ Size
General Medicine
Hydroxyproline
Aspergillus
medicine.anatomical_structure
Liver
Methionine Adenosyltransferase
Biotechnology
medicine.medical_specialty
CCL4
Spleen
Biology
Protective Agents
Collagen Type I
Transforming Growth Factor beta1
Internal medicine
Matrix Metalloproteinase 13
medicine
Animals
Aspartate Aminotransferases
RNA
Messenger
Rats
Wistar
Molecular Biology
Serum Albumin
Tissue Inhibitor of Metalloproteinase-1
Organic Chemistry
Tissue inhibitor of metalloproteinase
Rats
Collagen Type III
Endocrinology
chemistry
Fermentation
Carbon tetrachloride
Zdroj: Bioscience, Biotechnology, and Biochemistry. 71:1154-1161
ISSN: 1347-6947
0916-8451
DOI: 10.1271/bbb.60604
Popis: The purpose of this study was to investigate the hepatoprotective effects of a fermented substance from Aspergillus phoenicis (FSAP) on chronic liver injuries induced by carbon tetrachloride (CCl(4)) in rats. CCl(4) (20%; 0.2 ml/100 g body weight) was given twice a week for 9 weeks, and the rats received FSAP throughout the whole experimental period. Plasma ALT and AST, spleen weight, and hepatic levels of lipid peroxidation and hydroxyproline were significantly lower in the rats treated with FSAP as compared to CCl(4) only. Liver pathology in the FSAP-treated rats was also improved. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) analysis showed that FSAP treatment increased the expression of matrix metalloproteinase 13 and decreased the expression of methionine adenosyltransferase 2A, collagen (alpha1)(I), collagen (alpha1)(III), transforming growth factor-beta1, and tissue inhibitor of metalloproteinase 1. These results clearly indicate that FSAP partially reduced the liver fibrosis in rats induced by CCl(4).
Databáze: OpenAIRE
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