Cloning, characterization, and mRNA expression analysis of novel human fetal cochlear cDNAs
| Autor: | J. ten Caat, T.J.R. van de Pol, G. van Duijnhoven, Mirjam W. J. Luijendijk, Hannie Kremer, A.I. den Hollander, V. van Limpt, Han G. Brunner, F.P.M. Cremers |
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| Rok vydání: | 2003 |
| Předmět: |
Candidate gene
DNA Complementary Molecular Sequence Data Gene Expression Biology Deafness Complementary DNA Genetics medicine otorhinolaryngologic diseases Neurosensory disorders [UMCN 3.3] Humans Nonsyndromic deafness Cloning Molecular Gene In Situ Hybridization Gene Library Expressed sequence tag Base Sequence cDNA library Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Chromosome Mapping Sequence Analysis DNA medicine.disease Cochlea Genetic defects of metabolism [UMCN 5.1] CDNA Subtraction Suppression subtractive hybridization |
| Zdroj: | Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 82, 480-90 Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 82, 4, pp. 480-90 |
| ISSN: | 0888-7543 |
| Popis: | Item does not contain fulltext To identify novel genes that are expressed specifically or preferentially in the cochlea, we constructed a cDNA library enriched for human cochlear cDNAs using a suppression subtractive hybridization technique. We analyzed 2640 clones by sequencing and BLAST similarity searches. One hundred and fifty-five different cDNA fragments mapped in nonsyndromic hearing impairment loci for which the causative gene has not been cloned yet. Approximately 30% of the clones show no similarity to any known human gene or expressed sequence tag (EST). Clones mapping in nonsyndromic deafness loci and a selection of clones that represent novel ESTs were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) of RNA derived from 12 human fetal tissues. Our data suggest that a quarter of the novel genes in our library are preferentially expressed in fetal cochlea. These may play a physiologically important role in the hearing process and represent candidate genes for hereditary hearing impairment. |
| Databáze: | OpenAIRE |
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