Protective Effect of Polyphenols from Glycyrrhiza glabra Against Oxidative Stress in Caco-2 Cells
| Autor: | Vincenzo Zappia, Alessandra Morana, Patrizia Galletti, Anna Salvatore, Stefania D'Angelo |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Antioxidant
Free Radicals medicine.medical_treatment antioxidant activity Medicine (miscellaneous) medicine.disease_cause Antioxidants chemistry.chemical_compound Phenols Cell Line Tumor Glycyrrhiza medicine Humans liquorice Food science Caco-2 cells Flavonoids chemistry.chemical_classification Reactive oxygen species Nutrition and Dietetics Glycyrrhiza glabra biology Plant Extracts polyphenol Extraction (chemistry) Polyphenols food and beverages biology.organism_classification Oxidative Stress Biochemistry chemistry Caco-2 Polyphenol Methanol Reactive Oxygen Species Oxidative stress |
| Zdroj: | Journal of medicinal food 12 (2009): 1326–1333. doi:10.1089/jmf.2008.0285 info:cnr-pdr/source/autori:D'Angelo S.; Morana A.; Salvatore A.; Zappia V.; Galletti P./titolo:Protective effect of polyphenols from %2FGlycyrrhiza glabra%2F against oxidative stress in Caco-2 cells/doi:10.1089%2Fjmf.2008.0285/rivista:Journal of medicinal food/anno:2009/pagina_da:1326/pagina_a:1333/intervallo_pagine:1326–1333/volume:12 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2008.0285 |
| Popis: | In the present article, we have investigated the antioxidant properties of methanolic liquorice polyphenol extracts (LPE(s)). Polyphenol extraction was performed with 60% and 100% methanol. Analysis of LPE(s) by thin-layer chromatography revealed that a higher amount of polyphenols was recovered by extraction with 60% methanol. Antioxidant activity measurement of the reducing power, scavenging effect on 2,2'-diphenyl-1-picrylhydrazyl free radical, and hydrogen peroxide scavenging capability have been taken as the parameters for assessment of antioxidant potential of LPE(s). Results have been compared with both natural and synthetic antioxidants. All experimental data have indicated that LPE(s) possess strong antioxidant power proportional to their o-diphenolic and total polyphenolic content, independently from the assay used. Therefore, the LPE(s) antioxidant property was examined against the cytotoxic effects of reactive oxygen species in human colon carcinoma cells. Pretreatment of Caco-2 cells with liquorice polyphenolic extracts provided a remarkable protection against oxidative damage induced by H(2)O(2). The highest oxidative stress protection (72% of cell vitality) was measured in cells pretreated with 0.54 mM polyphenols. This effect seems to be associated to the antioxidant activity of liquorice polyphenolic compounds. Our data suggest that polyphenols from Glycyrrhiza glabra could exert a beneficial action in the prevention of intestinal pathologies related to production of reactive oxygen species. |
| Databáze: | OpenAIRE |
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