Anticonvulsive and neuroprotective effects of aqueous and methanolic extracts of Anacyclus pyrethrum root in kainic acid-induced-status epilepticus in mice
| Autor: | Saâdia Ba-M’hamed, Houria Manouze, Zahra Sokar, Otmane Bouchatta, Mohamed Bennis |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Anacyclus pyrethrum Kainic acid medicine.medical_treatment Intraperitoneal injection Neurological disorder Status epilepticus Pharmacology Neuroprotection Mice 03 medical and health sciences chemistry.chemical_compound Epilepsy 0302 clinical medicine Excitatory Amino Acid Agonists medicine Animals Chrysanthemum cinerariifolium Kainic Acid biology Plant Extracts business.industry Methanol biology.organism_classification medicine.disease Disease Models Animal Neuroprotective Agents 030104 developmental biology Anticonvulsant Neurology chemistry Anticonvulsants Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Epilepsy Research. 158:106225 |
| ISSN: | 0920-1211 |
| Popis: | Epilepsy is a chronic neurological disorder characterized by spontaneous and recurrent seizures. The currently available synthetic antiepileptic drugs have a limited efficacy and are associated with a wide range of side effects. In Ayurveda, Anacyclus pyrethrum root (APR) has been used as a traditional antiepileptic remedy. The aim of the present study is to evaluate the anticonvulsive and neuroprotective effects of aqueous and methanol extracts of Anacyclus pyrethrum root (AEAPR and MEAPR) on experimental model of status epilepticus (SE).Twenty four male mice were divided into four groups. The control and KA groups had free access to tap water for 5 days before the intraperitoneal injection of distillated water or kainic acid (KA; 30 mg/kg), respectively. In the treated groups, mice received extracts solutions MEAPR and AEAPR in drinking water at the concentration of 5 g/l for 5 days. At the fifth day, animals received intraperitoneal injection of KA. The behavioral changes latency of seizures, the number of wet dog shakes (WDS) and the mortality were observed over 6 h. Thereafter, the mice were sacrificed for immunohistochemical studies.Pretreatment with MEAPR and AEAPR decreases significantly the frequency of WDS (32.5% and 43.9%, p 0.01; respectively), and increases considerably the latent period (77.9% and 91.9%, p0.01; respectively) between the injection of the KA and the appearance of the SE as compared to the KA group. The duration and severity of seizure in the MEAPR or AEAPR-pretreated groups were significantly lower (p 0.01 and p 0.05 or p 0.01; respectively) than those in the KA group. These behavioral results were confirmed by the immunohistochemical study at the level of the hippocampus, in which the c-FOS and GFAP expression of both MEAPR and AEAPR-treated animals largely reduced (p 0.001) the number of labelled cells with respect to the group, which received the KA alone.Our results showed that the MEAPR and AEAPR have anticonvulsive effect and putative neuroprotective effect against seizures induced by KA. Further studies are required to identify its active ingredients responsible for the observed effects. |
| Databáze: | OpenAIRE |
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