Reduced masticatory function is related to lower satellite cell numbers in masseter muscle
| Autor: | Stavros Kiliaridis, Sander Grefte, Mette A. R. Kuijpers, Ewald M. Bronkhorst, J.W. Von den Hoff, Carine Carels |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Collagen Type IV
Male Satellite Cells Skeletal Muscle Cell Population Cell Count Orthodontics Haematoxylin Rats Sprague-Dawley Masseter muscle chemistry.chemical_compound Neck Muscles medicine Animals education Frozen section procedure education.field_of_study Myosin Heavy Chains Masseter Muscle Other Research Radboud Institute for Health Sciences [Radboudumc 0] Soft diet PAX7 Transcription Factor Metabolic Disorders Radboud Institute for Molecular Life Sciences [Radboudumc 6] Anatomy ddc:617.6 Diet Masticatory force Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] medicine.anatomical_structure chemistry Control muscle |
| Zdroj: | European Journal of Orthodontics, 36, 262-7 The European Journal of Orthodontics, Vol. 36, No 3 (2014) pp. 262-267 European Journal of Orthodontics, 36, 3, pp. 262-7 |
| ISSN: | 0141-5387 |
| DOI: | 10.1093/ejo/cjs044 |
| Popis: | summary The physiology of masseter muscles is known to change in response to functional demands, but the effect on the satellite cell (SC) population is not known. In this study, the hypothesis is tested that a decreased functional demand of the masseter muscle causes a reduction of SCs. To this end, twelve 5-week-old male Sprague–Dawley rats were put on a soft diet (SD, n = 6) or a hard diet (HD, n = 6) and sacrificed after 14 days. Paraffin sections of the superficial masseter and the m. digastricus (control muscle) were stained with haematoxylin and eosin for tissue survey and with anti-myosin heavy chain (MHC) for slow and fast fibres. Frozen sections of both muscles were double-stained for collagen type IV and Pax7. Slow MHC fibres were equally distributed in the m. digastricus but only localized in a small area of the m. masseter. No differences between HD or SD for the m. digastricus were found. The m. masseter had more SCs per fibre in HD than in SD (0.093 ± 0.007 and 0.081 ± 0.008, respectively; P = 0.027). The m. masseter had more fibres per surface area than the m. digastricus in rats with an SD group (758.1 ± 101.6 and 568.4±85.6, P = 0.047) and a HD group (737.7 ± 32.6 and 592.2 ± 82.2; P = 0.007). The m. digastricus had more SCs per fibre than the m. masseter in the SD group (0.094 ± 0.01 and 0.081 ± 0.008; P = 0.039). These results suggest that reduced masseter muscle function is related to a lower number of SCs. Reduced muscle function might decrease microdamage and hence the requirement of SCs in the muscle fibres. |
| Databáze: | OpenAIRE |
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