Actions of gluco-oligosaccharide against Staphylococcus aureus
| Autor: | Tetsumasa Yamada, Huh Wk, Hideto Uemura, Keiji Iwatsuki, Osamu Yamasaki, Hisanori Akiyama, Takashi Oono, Yukie Akagi |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Staphylococcus aureus Sodium Colony Count Microbial chemistry.chemical_element Oligosaccharides Dermatology Microbial Sensitivity Tests Biology medicine.disease_cause Sensitivity and Specificity Microbiology Dermatitis Atopic Glycocalyx Reference Values medicine Humans Incubation chemistry.chemical_classification General Medicine Atopic dermatitis Oligosaccharide medicine.disease Culture Media chemistry Concanavalin A biology.protein Female Staphylococcal Skin Infections |
| Zdroj: | The Journal of dermatology. 29(9) |
| ISSN: | 0385-2407 |
| Popis: | Heavy colonization of atopic dermatitis (AD) with Staphylococcus aureus is well documented. The purpose of the present study is to examine the actions of gluco-oligosaccharide (G-OS) against S. aureus for the control of AD skin lesions infected with S. aureus. The colony counts of S. aureus cells in 0.5% sodium chloride solution supplemented with 5% G-OS (pH 4.8) were about 10-fold lower than those in 0.5% sodium chloride solution (pH 6.6; control) after incubation for 24 hours. The colony counts of S. aureus cells attached on the coverslips (pre-treatment with 1% and 5% G-OS/PBS and following treatment with plasma) were about 10-fold lower than those on the coverslips (pre-treatment with PBS and following treatment with plasma; control) in PBS after incubation for 24 hours. The materials (sugars, probably glycocalyx) that stained positively for fluorescein-isothiocyanate (FITC) -concanavalin A and were consistent with the presence of S. aureus cells were reduced when S. aureus cells attached to the coverslips treated with 5% GC-OS. In conclusion, C-OS is a promising agent that can be applied topically in a cream to clear adherent S. aureus cells from skin lesions of AD in order to prevent its exacerbation. Further, 5% C-OS can inhibit glycocalyx production by S. aureus cells and consequently have some suppressive effect on the colonization of S. aureus on the horny cells of AD lesions. |
| Databáze: | OpenAIRE |
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