Critical evaluation of the use of bioinformatics as a theoretical tool to find high-potential sources of ACE inhibitory peptides
Autor: | Lieselot Vercruysse, Maté Ongenaert, Guy Smagghe, Aart van Amerongen, John Van Camp, Arie van der Bent |
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Jazyk: | angličtina |
Rok vydání: | 2009 |
Předmět: |
separation
Physiology In silico Molecular Sequence Data pea hydrolysate Muscle Proteins Peptide Angiotensin-Converting Enzyme Inhibitors bombyx-mori Biology digestion Bioinformatics Inhibitory postsynaptic potential Biochemistry Homology (biology) whey-protein Cellular and Molecular Neuroscience Endocrinology Phylogenetics Bombyx mori Animals Amino Acid Sequence Actin Phylogeny chemistry.chemical_classification AFSG Food Quality fungi Computational Biology Angiotensin-converting enzyme sequence biology.organism_classification Bombyx Actins i-converting-enzyme chemistry skeletal-muscle proteins AFSG Biobased Products biology.protein Insect Proteins hydrolyzate Peptides |
Zdroj: | Peptides, 30(3, Special Issue), 575-582 Peptides 30 (2009) 3, Special Issue |
ISSN: | 0196-9781 |
Popis: | A bioinformatics analysis to screen for high-potential sources of angiotensin converting enzyme (ACE) inhibitory peptides was conducted in the area of insect muscle proteins. Vertebrate muscle proteins are reported as good sources of ACE inhibitory peptides, while the research on invertebrate muscle proteins is limited. A phylogenetic tree constructed with actin sequences of both vertebrate and invertebrate species indicated a high homology. Furthermore, a quantitative in silico ACE inhibition analysis suggested that actin proteins of invertebrates have potentials as new sources of ACE inhibitory peptides. On one insect, Bombyx mori, a more detailed in silico analysis was done followed by a small experimental study. The in silico analysis indicated B. mori as a high-potential source of ACE inhibitory peptides and this was supported by the ACE inhibitory activity of the partially purified actin preparation. In conclusion, in food science, in silico analysis can be used as fast initial screening tool to look for high-potential sources of ACE inhibitory peptides and other peptidic bioactivities. |
Databáze: | OpenAIRE |
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