Longitudinal analysis of ANA in the Systemic Lupus International Collaborating Clinics (SLICC) Inception Cohort
| Autor: | May Yee Choi, Ann Elaine Clarke, Murray Urowitz, John Hanly, Yvan St-Pierre, Caroline Gordon, Sang-Cheol Bae, Juanita Romero-Diaz, Jorge Sanchez-Guerrero, Sasha Bernatsky, Daniel J Wallace, David Isenberg, Anisur Rahman, Joan T Merrill, Paul R Fortin, Dafna D Gladman, Ian N Bruce, Michelle Petri, Ellen M Ginzler, Mary Anne Dooley, Rosalind Ramsey-Goldman, Susan Manzi, Andreas Jönsen, Graciela S Alarcón, Ronald F van Vollenhoven, Cynthia Aranow, Meggan Mackay, Guillermo Ruiz-Irastorza, Sam Lim, Murat Inanc, Ken Kalunian, Søren Jacobsen, Christine Peschken, Diane L Kamen, Anca Askanase, Jill P Buyon, Karen H Costenbader, Marvin J Fritzler |
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| Přispěvatelé: | Rheumatology, AII - Inflammatory diseases, Clinical Immunology and Rheumatology, AMS - Musculoskeletal Health |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Indirect
Lupus Erythematosus Systemic Clinical Sciences Immunology Fluorescent Antibody Technique Lupus Autoimmunity Systemic Lupus Erythematosus Autoimmune Disease General Biochemistry Genetics and Molecular Biology Article Antibodies Arthritis & Rheumatology Cross-Sectional Studies Rheumatology Antibodies Antinuclear Antinuclear Public Health and Health Services Immunology and Allergy Lupus Erythematosus Systemic Humans skin and connective tissue diseases Fluorescent Antibody Technique Indirect Autoantibodies |
| Zdroj: | Annals of the Rheumatic Diseases, 81(8), 1143-1150. BMJ Publishing Group Annals of the rheumatic diseases, vol 81, iss 8 Choi, M Y, Clarke, A E, Urowitz, M, Hanly, J, St-Pierre, Y, Gordon, C, Bae, S C, Romero-Diaz, J, Sanchez-Guerrero, J, Bernatsky, S, Wallace, D J, Isenberg, D, Rahman, A, Merrill, J T, Fortin, P R, Gladman, D D, Bruce, I N, Petri, M, Ginzler, E M, Dooley, M A, Ramsey-Goldman, R, Manzi, S, Jönsen, A, Alarcón, G S, Van Vollenhoven, R F, Aranow, C, Mackay, M, Ruiz-Irastorza, G, Lim, S, Inanc, M, Kalunian, K, Jacobsen, S, Peschken, C, Kamen, D L, Askanase, A, Buyon, J P, Costenbader, K H & Fritzler, M J 2022, ' Longitudinal analysis of ANA in the Systemic Lupus International Collaborating Clinics (SLICC) Inception Cohort ', Annals of the Rheumatic Diseases, vol. 81, no. 8, pp. 1143-1150 . https://doi.org/10.1136/annrheumdis-2022-222168 Choi, M Y, Clarke, A E, Urowitz, M, Hanly, J, St-Pierre, Y, Gordon, C, Bae, S-C, Romero-Diaz, J, Sanchez-Guerrero, J, Bernatsky, S, Wallace, D J, Isenberg, D, Rahman, A, Merrill, J T, Fortin, P R, Gladman, D D, Bruce, I N, Petri, M, Ginzler, E M, Dooley, M A, Ramsey-Goldman, R, Manzi, S, Jönsen, A, Alarcón, G S, van Vollenhoven, R F, Aranow, C, Mackay, M, Ruiz-Irastorza, G, Lim, S, Inanc, M, Kalunian, K, Jacobsen, S R, Peschken, C, Kamen, D L, Askanase, A, Buyon, J P, Costenbader, K H & Fritzler, M J 2022, ' Longitudinal analysis of ANA in the Systemic Lupus International Collaborating Clinics (SLICC) Inception Cohort ', Annals of the Rheumatic Diseases, vol. 81, no. 8, pp. 1143-1150 . https://doi.org/10.1136/annrheumdis-2022-222168 Ann Rheum Dis Annals of the rheumatic diseases, 81(8), 1143-1150. BMJ Publishing Group |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/annrheumdis-2022-222168 |
| Popis: | ObjectivesA perception derived from cross-sectional studies of small systemic lupus erythematosus (SLE) cohorts is that there is a marked discrepancy between antinuclear antibody (ANA) assays, which impacts on clinicians’ approach to diagnosis and follow-up. We compared three ANA assays in a longitudinal analysis of a large international incident SLE cohort retested regularly and followed for 5 years.MethodsDemographic, clinical and serological data was from 805 SLE patients at enrolment, year 3 and 5. Two HEp-2 indirect immunofluorescence assays (IFA1, IFA2), an ANA ELISA, and SLE-related autoantibodies were performed in one laboratory. Frequencies of positivity, titres or absorbance units (AU), and IFA patterns were compared using McNemar, Wilcoxon and kappa statistics, respectively.ResultsAt enrolment, ANA positivity (≥1:80) was 96.1% by IFA1 (median titre 1:1280 (IQR 1:640–1:5120)), 98.3% by IFA2 (1:2560 (IQR 1:640–1:5120)) and 96.6% by ELISA (176.3 AU (IQR 106.4 AU–203.5 AU)). At least one ANA assay was positive for 99.6% of patients at enrolment. At year 5, ANA positivity by IFAs (IFA1 95.2%; IFA2 98.9%) remained high, while there was a decrease in ELISA positivity (91.3%, p91% agreement in ANA positivity at all time points and ≥71% agreement in IFA patterns between IFA1 and IFA2.ConclusionIn recent-onset SLE, three ANA assays demonstrated commutability with a high proportion of positivity and titres or AU. However, over 5 years follow-up, there was modest variation in ANA assay performance. In clinical situations where the SLE diagnosis is being considered, a negative test by either the ELISA or HEp-2 IFA may require reflex testing. |
| Databáze: | OpenAIRE |
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