Sortase-Mediated Ligation of Purely Artificial Building Blocks
| Autor: | Ulrich Glebe, Diana M. Mate, Andrea Körner, Dai Xiaolin, Alexander Böker, Tayebeh Mirzaei Garakani, Ulrich Schwaneberg |
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| Přispěvatelé: | Alexander von Humboldt Foundation, European Commission, Staatskanzlei des Landes Nordrhein-Westfalen, Publica |
| Rok vydání: | 2017 |
| Předmět: |
Polymers and Plastics
enzymes Nanoparticle Peptide 010402 general chemistry 01 natural sciences Article Sortase-mediated ligation lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Recognition sequence Dynamic light scattering Sortase Copolymer sortase-mediated ligation block copolymers nanoparticles chemistry.chemical_classification 010405 organic chemistry General Chemistry Combinatorial chemistry Block copolymers 0104 chemical sciences Enzymes chemistry Sortase A ddc:540 Nanoparticles Institut für Chemie Ethylene glycol |
| Zdroj: | Polymers Digital.CSIC. Repositorio Institucional del CSIC instname Polymers; Volume 10; Issue 2; Pages: 151 Polymers, Vol 10, Iss 2, p 151 (2018) |
| ISSN: | 2073-4360 |
| Popis: | Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated. EU and the federal state of North Rhine–Westphalia (grant EFRE 30 00 883 02) |
| Databáze: | OpenAIRE |
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