Biocompatible curcumin loaded PMMA-PEG/ZnO nanocomposite induce apoptosis and cytotoxicity in human gastric cancer cells
| Autor: | Jothi Ranjani, Patrick K. Bowen, Jamespandi Annaraj, Jeyaprakash Rajendhran, Jeyanthinath Mayandi, Raman Dhivya |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Curcumin Metal Nanoparticles Bioengineering Nanotechnology Apoptosis 02 engineering and technology 010402 general chemistry 01 natural sciences Nanocomposites Polyethylene Glycols Biomaterials chemistry.chemical_compound Stomach Neoplasms Humans Polymethyl Methacrylate Viability assay Particle Size Cytotoxicity Liposome technology industry and agriculture 021001 nanoscience & nanotechnology Biodegradable polymer 0104 chemical sciences Bioavailability chemistry Mechanics of Materials Cancer cell Drug delivery Biophysics 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 80 |
| ISSN: | 1873-0191 |
| Popis: | Although curcumin is efficient in killing cancer cells, its poor water solubility and assocaited inadequate bioavailability remain major limitations to its therapeutic application. The formulation of curcumin micellar nanoparticles (NPs) encapsulated with a biodegradable polymer promises to significantly improve curcumin's solubility, stability, and bioavailability. The past decade has witnessed the development of nanoscale curcumin delivery systems: curcumin-loaded liposomes or nanoparticles, self-microemulsifying drug delivery systems (SMEDDS), cyclodextrin inclusions, solid dispersions, nanodisks, and nanotubes. The intention of the present investigation was to enhance the bioavailability and ultimately the efficacy of curcumin by developing a curcumin loaded PMMA-PEG/ZnO bionanocomposite utilizing insoluble curcumin and poorly soluble ZnO nanoparticles. Here, the drug (curcumin) may be carry and deliver the biomolecule(s) by polymer-encapsulated ZnO NPs. Physical characteristics of these novel nanomaterials have been studied with transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) in conjunction with spectral techniques. Aqueous solubility of curcumin was augmented upon conjugation with the polymer-stabilized ZnO NPs. A narrow nanocomposite particle size distribution with an average value of 40 to 90nm was found via TEM. Most importantly, the pH-responsive release of curcumin from the nano-vehicle ensures safer, more controlled delivery of the drug at physiological pH. Cytotoxic potential and cellular uptake of curcumin loaded ZnO NPs were assessed by) cell viability assay, cell cycle assays along with the cell imaging studies have been done in addition to MTT using AGS cancer cells. Hence, these studies demonstrate that the clinical potential of the Curcumin Loaded PMMA-PEG/ZnO can induce the apoptosis of cancer cells through a cell cycle mediated apoptosis corridor, which raises its probability to cure gastric cancer cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect