Expression of adhesin genes and biofilm formation among Klebsiella oxytoca clinical isolates from patients with antibiotic-associated haemorrhagic colitis
| Autor: | Suhad Saad Mahmood, Ashraf Mohabati Mobarez, Abdolmajid Ghasemian, Sepideh Khodaparast, Shahin Najar Peerayeh, Amin Talebi Bezmin Abadi |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Adolescent medicine.drug_class 030106 microbiology Antibiotics Virulence Drug resistance Real-Time Polymerase Chain Reaction Microbiology Feces Young Adult 03 medical and health sciences medicine Humans Adhesins Bacterial Child Gene Aged Aged 80 and over biology Klebsiella oxytoca Biofilm Infant Gene Expression Regulation Bacterial General Medicine Middle Aged biochemical phenomena metabolism and nutrition Colitis biology.organism_classification Anti-Bacterial Agents Klebsiella Infections Bacterial adhesin 030104 developmental biology Biofilms Child Preschool Colistin Female Gastrointestinal Hemorrhage medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 68:978-985 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.000965 |
| Popis: | Purpose. Biofilm formation and resistance to last-line antibiotics have restricted chemotherapy options toward infection eradication. Methodology. Fifty K. oxytoca isolates were collected from patients with antibiotic-associated haemorrhagic colitis (AAHC). Antibiotic susceptibility tests were conducted and phenotypic biofilm formation was assessed using microtitre tissue plate (MTP) assay. PCR was employed to amplify the adhesins, extended-spectrum β-lactamases (ESBLs), carbapenemase and colistin resistance genes. The expression of adhesin genes was evaluated using quantitative real-time PCR (RT-qPCR). Results/Key findings. The previous antibiotic consumption and hospitalization (P |
| Databáze: | OpenAIRE |
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