Activity-Dependent Dendritic Arborization Mediated by CaM-Kinase I Activation and Enhanced CREB-Dependent Transcription of Wnt-2
| Autor: | Victor A. Derkach, Gary A. Wayman, Wilmon F. Grant, Thomas R. Soderling, Takeo Saneyoshi, Daniel L. Marks, Soren Impey |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
MAPK/ERK pathway
Transcription Genetic Neuroscience(all) DEVBIO CREB Hippocampus MOLNEURO Wnt2 Protein Rats Sprague-Dawley 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine Ca2+/calmodulin-dependent protein kinase Animals Premovement neuronal activity Cyclic AMP Response Element-Binding Protein Protein kinase A Transcription factor Cells Cultured 030304 developmental biology 0303 health sciences biology General Neuroscience Wnt signaling pathway Dendrites Rats Cell biology Enzyme Activation Animals Newborn Calcium-Calmodulin-Dependent Protein Kinase Type 1 nervous system SIGNALING Calcium-Calmodulin-Dependent Protein Kinases biology.protein Signal transduction Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuron. 50:897-909 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2006.05.008 |
| Popis: | SummaryMembers of the Wnt signaling family are important mediators of numerous developmental events, including activity-dependent dendrite development, but the pathways regulating expression and secretion of Wnt in response to neuronal activity are poorly defined. Here, we identify an NMDA receptor-mediated, Ca2+-dependent signaling pathway that couples neuronal activity to dendritic arborization through enhanced Wnt synthesis and secretion. Activity-dependent dendritic outgrowth and branching in cultured hippocampal neurons and slices is mediated through activation by CaM-dependent protein kinase kinase (CaMKK) of the membrane-associated γ isoform of CaMKI. Downstream effectors of CaMKI include the MAP-kinase pathway of Ras/MEK/ERK and the transcription factor CREB. A serial analysis of chromatin occupancy screen identified Wnt-2 as an activity-dependent CREB-responsive gene. Neuronal activity enhances CREB-dependent transcription of Wnt-2, and expression of Wnt-2 stimulates dendritic arborization. This novel signaling pathway contributes to dynamic remodeling of the dendritic architecture in response to neuronal activity during development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|