Fibrinolysis as a target to enhance osteoporotic fracture healing by vibration therapy in a metaphyseal fracture model
| Autor: | Jie Li, Victoria Man Huen Choy, Simon Kwoon-Ho Chow, Kwok-Sui Leung, Ronald Man Yeung Wong, Yu Ning Chim, Jack C. Y. Cheng, Wing-Hoi Cheung, Tsz Kiu Li, Meng Chen Michelle Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Orthodontics
lcsh:Diseases of the musculoskeletal system 060102 archaeology business.industry medicine.medical_treatment 030209 endocrinology & metabolism 06 humanities and the arts Bone healing vibration therapy osteoporotic fracture 03 medical and health sciences 0302 clinical medicine Bone Fracture Fibrinolysis Medicine 0601 history and archaeology Orthopedics and Sports Medicine Surgery Osteoporotic fracture fibrinolysis lcsh:RC925-935 business metaphyseal fracture lmhfv Metaphyseal fracture |
| Zdroj: | Bone & Joint Research, Vol 10, Iss 1, Pp 41-50 (2021) Bone & Joint Research |
| ISSN: | 2046-3758 |
| DOI: | 10.1302/2046-3758.101.BJR-2020-0185.R1 |
| Popis: | Aims Fibrinolysis plays a key transition step from haematoma formation to angiogenesis and fracture healing. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical modality proven to enhance fibrinolytic factors. This study investigates the effect of LMHFV on fibrinolysis in a clinically relevant animal model to accelerate osteoporotic fracture healing. Methods A total of 144 rats were randomized to four groups: sham control; sham and LMHFV; ovariectomized (OVX); and ovariectomized and LMHFV (OVX-VT). Fibrinolytic potential was evaluated by quantifying fibrin, tissue plasminogen activator (tPA), and plasminogen activator inhibitor-1 (PAI-1) along with healing outcomes at three days, one week, two weeks, and six weeks post-fracture. Results All rats achieved healing, and x-ray relative radiopacity for OVX-VT was significantly higher compared to OVX at week 2. Martius Scarlet Blue (MSB) staining revealed a significant decrease of fibrin content in the callus in OVX-VT compared with OVX on day 3 (p = 0.020). Mean tPA from muscle was significantly higher for OVX-VT compared to OVX (p = 0.020) on day 3. Mechanical testing revealed the mean energy to failure was significantly higher for OVX-VT at 37.6 N mm (SD 8.4) and 71.9 N mm (SD 30.7) compared with OVX at 5.76 N mm (SD 7.1) (p = 0.010) and 17.7 N mm (SD 11.5) (p = 0.030) at week 2 and week 6, respectively. Conclusion Metaphyseal fracture healing is enhanced by LMHFV, and one of the important molecular pathways it acts on is fibrinolysis. LMHFV is a promising intervention for osteoporotic metaphyseal fracture healing. The improved mechanical properties, acceleration of fracture healing, and safety justify its role into translation to future clinical studies. Cite this article: Bone Joint Res 2021;10(1):41–50. |
| Databáze: | OpenAIRE |
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