Dextran-Curcumin Nanoparticles as a Methotrexate Delivery Vehicle: A Step Forward in Breast Cancer Combination Therapy
| Autor: | Annafranca Farfalla, Paola Tucci, Manuela Curcio, Fiore Pasquale Nicoletta, Emilia Bevacqua, Orazio Vittorio, Giuseppe Cirillo, Francesca Iemma |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Combination therapy Pharmaceutical Science Nanoparticle lcsh:Medicine lcsh:RS1-441 02 engineering and technology Article lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound polyphenol conjugate breast cancer Dynamic light scattering synergism Drug Discovery medicine curcumin skin and connective tissue diseases self-assembling nanoparticles lcsh:R 021001 nanoscience & nanotechnology 030104 developmental biology Dextran chemistry Curcumin Biophysics Molecular Medicine Methotrexate Nanocarriers 0210 nano-technology medicine.drug Conjugate |
| Zdroj: | Pharmaceuticals Volume 13 Issue 1 Pharmaceuticals, Vol 13, Iss 1, p 2 (2019) |
| ISSN: | 1424-8247 |
| DOI: | 10.3390/ph13010002 |
| Popis: | With the aim to effectively deliver methotrexate (MTX) to breast cancer cells, we designed a nanocarrier system (DC) derived from the self-assembly of a dextran-curcumin conjugate prepared via enzyme chemistry with immobilized laccase acting as a solid biocatalyst. Nanoparticles consisted of homogeneously dispersed nanospheres with a mean diameter of 290 nm, as characterized by combined transmission electron microscopy and dynamic light scattering investigations. DC was able to control the MTX release overtime (t1/2 value of 310 min), with cell internalization studies proving its presence inside MCF-7 cytoplasm. Finally, improved MTX efficacy was obtained in viability assays, and attributed to the synergy of curcumin moieties and loaded MTX as underlined by a combination index (CI) < 1. |
| Databáze: | OpenAIRE |
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