Improving performance of cell imprinted PDMS by integrating boronate affinity and local post-imprinting modification for selective capture of circulating tumor cells from cancer patients

Autor: Yi Sun, Yi Luo, Lu Sun, Xiao-Rui Wang, Li-Wei Chen, Ning Zhang, Yu Wang, Lin-Yi Dong, Hua Guo, Xian-Hua Wang
Rok vydání: 2023
Předmět:
Zdroj: Biosensors and Bioelectronics. 223:115023
ISSN: 0956-5663
DOI: 10.1016/j.bios.2022.115023
Popis: Efficient capture of circulating tumor cells (CTCs) from cancer patients is an important technique that may promote early diagnosis and prognosis monitoring of cancer. However, the existing systems have certain disadvantages, such as poor selectivity, low capture efficiency, consumption of antibodies, and difficulty in release of CTCs for downstream analysis. Herein, we fabricated an innovative PEGylated boronate affinity cell imprinted polydimethylsiloxane (PBACIP) for highly efficient capture of CTCs from cancer patients. The antibody-free PBACIP possessed hierarchical structure of imprinted cavities, which were inlaid with boronic acid modified SiO
Databáze: OpenAIRE
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