Perillyl alcohol and perillaldehyde induced cell cycle arrest and cell death in BroTo and A549 cells cultured in vitro
| Autor: | Robert C. Wang, J. Abiodun Elegbede, Richard Flores |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lung Neoplasms
Cell cycle checkpoint Apoptosis Phosphatidylserines Adenocarcinoma Biology General Biochemistry Genetics and Molecular Biology Cell Line chemistry.chemical_compound Tumor Cells Cultured Humans General Pharmacology Toxicology and Pharmaceutics Tumor Stem Cell Assay A549 cell Cell Death Cell growth Perillyl alcohol Cell Cycle General Medicine Cell cycle Molecular biology chemistry Head and Neck Neoplasms Cell culture Cancer cell Carcinoma Squamous Cell Monoterpenes Indicators and Reagents Oxidoreductases |
| Zdroj: | Life Sciences. 73:2831-2840 |
| ISSN: | 0024-3205 |
| Popis: | The role of the monoterpenes, especially limonene and perillyl alcohol, in the treatment of certain cancers is currently being evaluated in clinical trials. In this study, the effects of perillyl alcohol (POH) and its analog, perillaldehyde (PALD), on human carcinoma cell lines (BroTo and A549) cultured in vitro were investigated using proliferation assays (MTT and colony formation) and DNA content analysis by flow cytometry. POH and PALD elicited dose- and time-dependent inhibition of proliferation in both cell lines. Concentrations of POH and PALD that inhibited cell proliferation by 50% (IC50) in 24 hr were 1 and 3 mM, respectively. DNA content analysis revealed that 1 mM of either POH or PALD caused cell cycle arrest in the G1 phase in both cell lines while POH alone caused increased hypodiploid and annexin V-positive populations in both BroTo and A549 cells. POH induced apoptosis and was more effective than PALD at inhibiting the proliferation of BroTo and A549 cells cultured in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect