Induction of base-pair substitution and prameshift mutations in wild-type and repair-deficient strains of Salmonella typhimurium by the photodynamic action of methylene blue
| Autor: | F. Paula Imray, Donald G. MacPhee |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
DNA
Bacterial Salmonella typhimurium DNA Repair Light Transcription Genetic Ultraviolet Rays Health Toxicology and Mutagenesis Auxotrophy Reversion Biology medicine.disease_cause Frameshift mutation Genetics medicine Radiation Genetics Molecular Biology Recombination Genetic Mutation Base Sequence Strain (chemistry) Point mutation Wild type Molecular biology Methylene Blue Genetic Code Nucleotide excision repair |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 27:299-306 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(75)90286-9 |
| Popis: | Induction of back mutations to prototrophy by methylene blue (MB)-sensitized photodynamic (PD) treatment has been studied in wild-type and repair-deficient strains of Salmonella typhimurium carrying either the base-pair substitution mutation hisG46 or the frameshift mutation hisD3052. We found that reversion of the hisG46 mutation was increased in a strain carrying a uvrB deletion and decreased in a strain carrying a recA-type mutation. Reversion of the hisD3052 (frameshift) mutation, on the other hand, was decreased in both uvrB deletion and recA-type strains. The former results are consistent with the hypothesis that the majority of MB-sensitized PD-induced base-pair substitution mutations arise by a mechanism similar to that currently believed to be involved in UV mutagenesis. The latter results suggest that PD-induced frameshift mutations may arise in some other way, and two possible mechanisms involving sequential action of the excision repair and recombinational repair pathways are considered. |
| Databáze: | OpenAIRE |
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