HPLC determination of polyethylene glycol 400 in urine: oligomeric profile in healthy and celiac disease subjects
| Autor: | José B. Fariña, H Armas, Alexis Oliva |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Glutens Macromolecular Substances Clinical Biochemistry Urine Polyethylene glycol Oligomer High-performance liquid chromatography Coeliac disease Polyethylene Glycols Excretion chemistry.chemical_compound Reference Values PEG ratio medicine Humans Chromatography High Pressure Liquid Intestinal permeability Chromatography Biochemistry (medical) medicine.disease Diet Molecular Weight Celiac Disease chemistry Female |
| Zdroj: | Europe PubMed Central Scopus-Elsevier |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | The absorption of orally administered polyethylene glycol (PEG) has been used to assess intestinal permeability. We describe a simple HPLC technique to determine the oligomeric profile of PEG excreted in urine. We measured the total (%) PEG excreted in 6 h and the ratio of the four smallest oligomers to the three largest oligomers (expressed as mean percentages). The proposed method differentiates distinct groups of subjects with varying degrees of intestinal permeability detected by intestinal biopsy. The percent of PEG excreted and the oligomer ratio values for healthy subjects were, respectively, 30.1 +/- 3.87 and 0.35 +/- 0.03; for celiac patients on a gluten-free diet, 24.5 +/- 6.65 and 0.45 +/- 0.16; and for celiac patients, 15.0 +/- 5.93 and 1.12 +/- 0.55. |
| Databáze: | OpenAIRE |
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