Analysis of prostaglandins by electron-capture gas chromatography. I. Thermal decomposition of heptafluorobutyrate methyl esters of F1α and F2α
| Autor: | John B. Josimovich, Kathy D. Broskin, Monte J. Levitt |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Allylic rearrangement
Chromatography Gas Electron capture chemistry.chemical_element Electrons In Vitro Techniques Tritium Mass spectrometry Methylation Biochemistry Mass Spectrometry chemistry.chemical_compound Endocrinology Drug Stability Methods Organic chemistry Chromatography Diazomethane Microchemistry Thermal decomposition Temperature food and beverages Esters Fluorine Decomposition Butyrates chemistry Prostaglandins Solvents Gas chromatography |
| Zdroj: | Prostaglandins. 1:121-131 |
| ISSN: | 0090-6980 |
| DOI: | 10.1016/0090-6980(72)90075-5 |
| Popis: | Triheptafluorobutyrate methyl ester (THFB-Met) derivatives are easily prepared from prostaglandins F1α and F2α by successive methylation and heptafluorobutyrylation. The derivatives are reasonably stable during storage, are volatile, and can be detected in the picogram range by electron-capture gas chromatography. Both derivatives exhibit peak broadening or multiple peak formation during gas chromatography at 190°–210°C. Decomposition is independent of the nature of the stationary phase and can be increased by prior heating. Studies with other derivatives suggest that thermal decomposition of the THFB-Met derivatives occuring during gas chromatography involves loss of a heptafluorobutyrate group from the allylic position 15 of the prostaglandins. |
| Databáze: | OpenAIRE |
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