In Vitro Assay for Quantifying Clumping of Staphylococcus aureus

Autor:	Alexander R. Horswill, Jakub Kwiecinski, Heidi A. Crosby
Rok vydání:	2021
Předmět:	Coagulase Staphylococcus aureus Chromatography Chemistry Fibrinogen In Vitro Techniques medicine.disease_cause Article Clumping factor A In vitro Slide agglutination Bacterial adhesin Agglutination (biology) Agglutination Tests medicine Humans Rabbit plasma medicine.drug
Zdroj:	Methods in Molecular Biology ISBN: 9781071615492 Methods Mol Biol
DOI:	10.1007/978-1-0716-1550-8_5
Popis:	Staphylococcus aureus interacts with fibrinogen in plasma to form macroscopic clumps of cells. A simple and rapid slide agglutination test using rabbit plasma has been employed in clinical labs to distinguish S. aureus from most coagulase negative Staphylococci. The method described here is a quantitative clumping assay in which S. aureus cells are mixed with either plasma or purified fibrinogen, and clumps are allowed to sediment out of solution. Clearing of the overlying solution is monitored over time by measuring the optical density at 600 nm and comparing these values to the initial turbidity. This simple assay can be used to study regulation and expression of various cell wall-anchored adhesins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad5ab890e63ac7820afd37a55aa1fd1c https://doi.org/10.1007/978-1-0716-1550-8_5 Zobrazit plný text záznamu