Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
| Autor: | Sebastián Pita, Analía Del Valle Garnero, Ricardo José Gunski, Tiago Marafiga Degrandi, Yanina Panzera, Lúcia Vinadé, Edivaldo Herculano Corrêa de Oliveira, José Ribamar Felipe Marques, M. R. Figueiro, Larissa Coelho Marques |
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| Přispěvatelé: | Tiago Marafiga Degrandi, Universidade Federal do Pampa, Sebastian Pita, University of the Republic, Montevideo, Uruguay., Yanina Panzera, University of the Republic, Montevideo, Uruguay., Edivaldo Herculano C. de Oliveira, UFPA / Institute Evandro Chagas, JOSE RIBAMAR FELIPE MARQUES, CPATU, MARIVALDO RODRIGUES FIGUEIRO, CPATU, Larissa Coêlho Marques, UFPA, Lucia Vinadé, Universidade Federal do Pampa, Ricardo José Gunski, Universidade Federal do Pampa, Analía Del Valle Garnero, Universidade Federal do Pampa. |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
lcsh:QH426-470
Bubalino Citogenética Zoology Chromosome 9 Biology Subspecies Crossbreed Swamp FISH parasitic diseases Bubalinos Genetics Molecular Biology Hybrid geography geography.geographical_feature_category 18S rDNA Genética Chromosome Karyotype DNA Ribosomal RNA Evolução cariotípica Bubalus bubalis lcsh:Genetics Animal Genetics Research Article |
| Zdroj: | Genetics and Molecular Biology, Vol 37, Iss 2, Pp 375-380 (2014) Genetics and Molecular Biology, Volume: 37, Issue: 2, Pages: 375-380, Published: JUN 2014 Genetics and Molecular Biology Genetics and Molecular Biology v.37 n.2 2014 Sociedade Brasileira de Genética (SBG) instacron:SBG Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Repositório Institucional da UFPA Universidade Federal do Pará (UFPA) instacron:UFPA |
| ISSN: | 1678-4685 |
| Popis: | DOMESTIC BUFFALOES ARE DIVIDED INTO TWO GROUP BASED ON CYTOGENETIC CHARACTERISTICS AND HABITATS: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 cross-breed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies. |
| Databáze: | OpenAIRE |
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