Benznidazole treatment in chronic children infected with Trypanosoma cruzi: serological and molecular follow-up of patients and identification of Discrete Typing Units
| Autor: | Julio R. Nasser, Rubén O. Cimino, Viviana Orellana, Nicolás Tomasini, Miguel A. Basombrío, Juan José Lauthier, José Fernando Gil, C.D. Lacunza, Patricio Diosque, A. M. Alberti d'Amato, C.M. Pérez Brandán, M. Monje Rumi, Paula G. Ragone |
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| Rok vydání: | 2012 |
| Předmět: |
Chagas disease
Male Rural Population Adolescent Genotype Veterinary (miscellaneous) Trypanosoma cruzi Antiprotozoal Agents Argentina Antibodies Protozoan Serology medicine Humans Chagas Disease Typing Seroconversion Child biology DNA Kinetoplast Nucleic Acid Hybridization DNA Protozoan biology.organism_classification medicine.disease Virology Hypervariable region Infectious Diseases Treatment Outcome Benznidazole Nitroimidazoles Insect Science Child Preschool Chronic Disease Parasitology Female medicine.drug Follow-Up Studies |
| Zdroj: | Acta tropica. 128(1) |
| ISSN: | 1873-6254 |
| Popis: | A total of 221 children from two rural settlements in Northeast Argentina were examined for T. cruzi infection. Blood samples were taken for serology tests and PCR assays. In addition, T. cruzi Discrete Typing Units (DTUs) were determined by hybridization with specific DNA probes of the minicircle hypervariable regions (mHVR). Serological results indicated that 26% (57/215) were reactive against T. cruzi antigens. PCR analyses were performed on seropositive samples showing presence of parasite DNA in 31 out of 53 samples (58.5%). All seropositive children underwent specific chemotherapy with Benznidazole (5mg/kg/day) for a period of two months and were monitored two and five years after treatment. Overall the treatment was well tolerated and low side effects were observed. Serological conversion was observed at two years post -treatment in one child form Pampa Avila and at five years in two children from Tres Estacas. However, at the end of the follow-up period, T. cruzi DNA could not be detected by PCR in samples from treated children, except in two cases. In addition, the results of hybridizations with specific DNA probes showed that DTU TcV was detected in 68% (21/31), TcVI in 7% (2/31) and TcV/VI in 3% (1/31) of the samples. Altogether, results of the follow-up of treated children showed a low rate of seroconversion; however trend toward seroconversion was evident at five years post-treatment. On the other hand, detection of T. cruzi DNA by PCR significantly decreased after Benznidazole treatment. The existence of data regarding serological and molecular follow-ups from controlled studies in the Chaco Region will be important for future treatment efforts against T. cruzi infection in this region. The results obtained in the present study represent a contribution in this regard. |
| Databáze: | OpenAIRE |
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