Comparison of interleukin-6 and matrix metalloproteinase expression in the subretinal fluid and the vitreous during proliferative vitreoretinopathy: Correlations with extent, duration of RRD and PVR grade

Autor: Ilias Georgalas, Tryfon Rotsos, Stavros A. Dimitrakos, Sofia Androudi, George Karakiulakis, Eudoxia Diza, Eleni Papakonstantinou, Chrysanthos Symeonidis
Rok vydání: 2014
Předmět:
Adult
Pars plana
Proliferative vitreoretinopathy
medicine.medical_specialty
genetic structures
medicine.medical_treatment
Immunology
Gelatin Zymography
Enzyme-Linked Immunosorbent Assay
Context (language use)
Vitrectomy
Matrix metalloproteinase
Biochemistry
Ophthalmology
Humans
Immunology and Allergy
Medicine
Interleukin 6
Molecular Biology
Tissue Inhibitor of Metalloproteinase-1
biology
Interleukin-6
business.industry
Subretinal Fluid
Vitreoretinopathy
Proliferative
Retinal Detachment
Hematology
medicine.disease
Matrix Metalloproteinases
eye diseases
Pathophysiology
Vitreous Body
Matrix Metalloproteinase 8
medicine.anatomical_structure
Matrix Metalloproteinase 9
biology.protein
Gelatin
Matrix Metalloproteinase 2
Electrophoresis
Polyacrylamide Gel
Matrix Metalloproteinase 3
sense organs
Matrix Metalloproteinase 1
business
Zdroj: Cytokine. 67:71-76
ISSN: 1043-4666
Popis: Introduction The full extent of IL-6 involvement in PVR pathophysiology has not yet been comprehensively investigated. The aim of this study was the comparison of the IL-6 effect on MMP expression between SRF and the vitreous in the context of RRD complicated by PVR. Materials and methods Thirty-one SRF samples from 31 eyes of 31 consecutive patients suffering from RRD with PVR were collected during treatment by scleral buckling. Twenty-eight vitreous samples from 28 eyes of 28 RRD patients with PVR were collected during surgical management with pars plana vitrectomy (PPV). Enzyme Linked Immunosorbent Assay was employed for the measurement of MMP-1, -3, -8 and TIMP-1 concentrations (in ng/ml). MMP gelatinolytic activity was determined with the use of gelatin zymography analysis using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Results Correlation analysis in the SRF revealed a significant correlation between MMP-1/IL6 and RRD duration. Regression analysis in the SRF revealed a significant correlation between the MMP-9/IL-6 and RRD extent. In the same fluid, with respect to PVR grade, ANOVA revealed a significant relationship with the proMMP-2/IL-6, MMP-2/IL6 and TIMP-1/IL-6 ratios. Graphical representation of the results revealed that, between the SRF and vitreous groups, significant peak values were observed for all MMP/IL-6 and TIMP-1/IL-6 ratios included in this study with the exception of the MMP-2/IL-6 ratio. Conclusions It appears that there is a significant correlation between the presence of IL-6 and MMP/TIMP ratio in the SRF, indicating that IL-6 may contribute to the increased MMP/TIMP ratio during PVR.
Databáze: OpenAIRE
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