Adsorption of Selenite onto Bacillus subtilis: The Overlooked Role of Cell Envelope Sulfhydryl Sites in the Microbial Conversion of Se(IV)
| Autor: | Maxim I. Boyanov, Jeremy B. Fein, Jinling Liu, Qiang Yu, Kenneth M. Kemner |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microorganism chemistry.chemical_element Bacillus subtilis 010501 environmental sciences Selenious Acid 01 natural sciences Cell wall Selenium 03 medical and health sciences Sodium Selenite Adsorption Cell Wall Oxidizing agent Environmental Chemistry 0105 earth and related environmental sciences biology Chemistry General Chemistry biology.organism_classification 030104 developmental biology Biochemistry Cell envelope Bacteria |
| Zdroj: | Environmental Science & Technology. 52:10400-10407 |
| ISSN: | 1520-5851 0013-936X |
| Popis: | Microbial activities play a central role in the global cycling of selenium. Microorganisms can reduce, methylate, and assimilate Se, controlling the transport and fate of Se in the environment. However, the mechanisms controlling these microbial activities are still poorly understood. In particular, it is unknown how the negatively charged Se(IV) and Se(VI) oxyanions that dominate the aqueous Se speciation in oxidizing environments bind to negatively charged microbial cell surfaces in order to become bioavailable. Here, we show that the adsorption of selenite onto Bacillus subtilis bacterial cells is controlled by cell envelope sulfhydryl sites. Once adsorbed onto the bacteria, selenite is reduced and forms reduced organo-Se compounds (e.g., R1S–Se–SR2). Because sulfhydryl sites are present within cell envelopes of a wide range of bacterial species, sulfhydryl-controlled adsorption of selenite likely represents a general mechanism adopted by bacteria to make selenite bioavailable. Therefore, sulfhydryl bi... |
| Databáze: | OpenAIRE |
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