Quantification of microbial uptake of quercetin and its derivatives using an UHPLC-ESI-QTOF mass spectrometry assay
| Autor: | Inamullah Hakeem Said, Nikolai Kuhnert, Rohan L. Shah, Matthias S. Ullrich |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Atmosphere Exposure Chambers Spectrometry Mass Electrospray Ionization Rutin Electrospray ionization ved/biology.organism_classification_rank.species Porins medicine.disease_cause Models Biological Antioxidants 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Glucosides Escherichia coli medicine Protein Isoforms Phenols Chromatography High Pressure Liquid Chromatography Bifidobacterium bifidum biology ved/biology General Medicine biology.organism_classification Absorption Physiological Oxygen Kinetics 030104 developmental biology chemistry Biochemistry Mutation Quercetin Efflux Multidrug Resistance-Associated Proteins Oxidation-Reduction Gene Deletion Bacteria Food Science |
| Zdroj: | Food & Function. 7:4082-4091 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/c6fo00652c |
| Popis: | A liquid chromatography-mass spectrometry-based method for the quantification of intracellular concentrations of dietary phenolics in bacteria was developed. Using this assay, the time-dependent uptake of quercetin and two of its glycosides into gut microbiota model organisms (Escherichia coli and Bifidobacterium bifidum) under aerobic and anaerobic conditions was studied. For the first time, quantitative data on the uptake of dietary phenols were obtained indicating a time-dependent differential uptake of the dietary compounds. The shape of the uptake curve and the comparative use of bacterial mutants lacking porins OmpFC or the multi-drug efflux pump AcrAB, respectively, along with the identification and quantification of selected bacterial metabolites provided a mechanistic insight into the uptake process. |
| Databáze: | OpenAIRE |
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